• Western blot analysis of extracts of various cell lines, using ALG1 antibody (STJ110128) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ALG1 antibody (215-464) (STJ110128)

SKU:
STJ110128

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALG1 (215-464) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALG1
Gene ID: 56052
Uniprot ID: ALG1_HUMAN
Immunogen Region: 215-464
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 215-464 of human ALG1 (NP_061982.3).
Immunogen Sequence: YDKPASFFKETPLDLQHRLF MKLGSMHSPFRARSEPEDPV TERSAFTERDAGSGLVTRLR ERPALLVSSTSWTEDEDFSI LLAALEKFEQLTLDGHNLPS LVCVITGKGPLREYYSRLIH QKHFQHIQVCTPWLEAEDYP LLLGSADLGVCLHTSSSGLD LPMKVVDMFGCCLPVCAVNF KCLHELVKHEENGLVFEDSE ELAAQLQMLFSNFPDPAGKL NQFRKNLRESQQLRWDESW
Function Catalyzes the addition of the first of nine mannose moieties to form a dolichol-lipid linked oligosaccharide intermediate required for proper N-linked glycosylation.
Protein Name Chitobiosyldiphosphodolichol Beta-Mannosyltransferase
Asparagine-Linked Glycosylation Protein 1 Homolog
Beta-1 -4-Mannosyltransferase
Gdp-Man -Glcnac2-Pp-Dolichol Mannosyltransferase
Gdp-Mannose-Dolichol Diphosphochitobiose Mannosyltransferase
Mannosyltransferase-1
Mt-1
Hmat-1
Database Links Reactome: R-HSA-446193
Reactome: R-HSA-4549380
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Chitobiosyldiphosphodolichol Beta-Mannosyltransferase antibody
Anti-Asparagine-Linked Glycosylation Protein 1 Homolog antibody
Anti-Beta-1 -4-Mannosyltransferase antibody
Anti-Gdp-Man -Glcnac2-Pp-Dolichol Mannosyltransferase antibody
Anti-Gdp-Mannose-Dolichol Diphosphochitobiose Mannosyltransferase antibody
Anti-Mannosyltransferase-1 antibody
Anti-Mt-1 antibody
Anti-Hmat-1 antibody
Anti-ALG1 antibody
Anti-HMAT1 antibody
Anti-HMT1 antibody
Anti-PSEC0061 antibody
Anti-UNQ861 antibody
Anti-PRO1870 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance