• Immunofluorescence analysis of A549 cells using ALDOA antibody (STJ22584). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ALDOA antibody (STJ22584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 2s.

Anti-ALDOA antibody (1-348) (STJ22584)

SKU:
STJ22584

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALDOA (1-348) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALDOA
Gene ID: 226
Uniprot ID: ALDOA_HUMAN
Immunogen Region: 1-348
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human ALDOA (NP_908930.1).
Immunogen Sequence: MPYQYPALTPEQKKELSDIA HRIVAPGKGILAADESTGSI AKRLQSIGTENTEENRRFYR QLLLTADDRVNPCIGGVILF HETLYQKADDGRPFPQVIKS KGGVVGIKVDKGVVPLAGTN GETTTQGLDGLSERCAQYKK DGADFAKWRCVLKIGEHTPS ALAIMENANVLARYASICQQ NGIVPIVEPEILPDGDHDLK RCQYVTEKVLAAVYKALSDH HIYLEGTLLKPNMVTPGHA
Function Catalyzes the reversible conversion of beta-D-fructose 1,6-bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
Protein Name Fructose-Bisphosphate Aldolase A
Lung Cancer Antigen Ny-Lu-1
Muscle-Type Aldolase
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-6798695
Reactome: R-HSA-70171
Reactome: R-HSA-70263
Cellular Localisation Cytoplasm
Myofibril
Sarcomere
I Band
M Line
In Skeletal Muscle
Accumulates Around The M Line And Within The I Band
Colocalizing With Fbp2 On Both Sides Of The Z Line In The Absence Of Ca(2+)
Alternative Antibody Names Anti-Fructose-Bisphosphate Aldolase A antibody
Anti-Lung Cancer Antigen Ny-Lu-1 antibody
Anti-Muscle-Type Aldolase antibody
Anti-ALDOA antibody
Anti-ALDA antibody

Information sourced from Uniprot.org

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