| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-AKR1C1 (1-323) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:3000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
AKR1C1 |
| Gene ID: |
1645 |
| Uniprot ID: |
AK1C1_HUMAN |
| Immunogen Region: |
1-323 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human AKR1C1 (NP_001344.2). |
| Immunogen Sequence: |
MDSKYQCVKLNDGHFMPVLG FGTYAPAEVPKSKALEATKL AIEAGFRHIDSAHLYNNEEQ VGLAIRSKIADGSVKREDIF YTSKLWCNSHRPELVRPALE RSLKNLQLDYVDLYLIHFPV SVKPGEEVIPKDENGKILFD TVDLCATWEAVEKCKDAGLA KSIGVSNFNRRQLEMILNKP GLKYKPVCNQVECHPYFNQR KLLDFCKSKDIVLVAYSALG SHREEPWVDPNSPVLLEDP |
| Tissue Specificity | Expressed in all tissues tested including liver, prostate, testis, adrenal gland, brain, uterus, mammary gland and keratinocytes. Highest levels found in liver, mammary gland and brain. |
| Function | Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH. Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens. May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate. Displays affinity for bile acids. |
| Protein Name | Aldo-Keto Reductase Family 1 Member C1 20-Alpha-Hydroxysteroid Dehydrogenase20-Alpha-HsdChlordecone Reductase Homolog HakrcDihydrodiol Dehydrogenase 1Dd1High-Affinity Hepatic Bile Acid-Binding ProteinHbab |
| Database Links | Reactome: R-HSA-193368Reactome: R-HSA-193775Reactome: R-HSA-193807Reactome: R-HSA-975634Reactome: R-HSA-9757110 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Aldo-Keto Reductase Family 1 Member C1 antibodyAnti-20-Alpha-Hydroxysteroid Dehydrogenase antibodyAnti-20-Alpha-Hsd antibodyAnti-Chlordecone Reductase Homolog Hakrc antibodyAnti-Dihydrodiol Dehydrogenase 1 antibodyAnti-Dd1 antibodyAnti-High-Affinity Hepatic Bile Acid-Binding Protein antibodyAnti-Hbab antibodyAnti-AKR1C1 antibodyAnti-DDH antibodyAnti-DDH1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and ERCC1 knockout (KO) HeLa cells, using [KO Validated] ERCC1 Rabbit polyclonal antibody (STJ11100039) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89111/357565/STJ11100039_1__56308.1713121691.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ERCC1 knockout (KO) HeLa cells, using [KO Validated] ERCC1 Rabbit polyclonal antibody (STJ11100039) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
