• Western blot analysis of lysates from mouse heart and Jurkat cells, using AKAP8 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human heart tissue, using AKAP8 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of MCF7 cells using AKAP 95 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunofluorescence analysis of HUVEC cells, using AKAP8 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using AKAP 95 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-AKAP8 antibody (331-380 aa) (STJ91533)

SKU:
STJ91533

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-A-kinase anchor protein 8 (331-380 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKAP8
Gene ID: 10270
Uniprot ID: AKAP8_HUMAN
Immunogen Region: 331-380 aa
Specificity: AKAP 95 Polyclonal Antibody detects endogenous levels of AKAP 95 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human AKAP8 at the amino acid range 331-380
Post Translational Modifications Phosphorylated on tyrosine residues probably by SRC subfamily protein kinases.multiple phosphorylation is leading to dissociation from nuclear structures implicated in chromatin structural changes.
Function Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation.the function appears to be independent from PKA-anchoring. May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression. Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3.in this function may act redundantly with AKAP8L. Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation. May be involved in regulation of DNA replication by acting as scaffold for MCM2. Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 'Lys-4' methylation. May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells. May act as a carrier protein of GJA1 for its transport to the nucleus. May play a repressive role in the regulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro. In cells, associates with ribosomal RNA (rRNA) chromatin, preferentially with rRNA promoter and transcribed regions. Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation.the function probably implicates targeting of PKA to NFKB1.
Protein Name A-Kinase Anchor Protein 8
Akap-8
A-Kinase Anchor Protein 95 Kda
Akap 95
Cellular Localisation Nucleus
Nucleus Matrix
Nucleolus
Cytoplasm
Associated With The Nuclear Matrix In Interphase And Redistributes Mostly To Chromatin At Mitosis
However
Mitotic Chromatin Localization Has Been Questioned
Upon Nuclear Reassembly At The End Of Mitosis
Is Sequestered Into The Daughter Nuclei Where It Re-Acquires An Interphase Distribution
Exhibits Partial Localization To The Nucleolus In Interphase
Where It Colocalizes With Ubtf/Ubf
Suggesting Localization To The Fibrillary Center And/Or To The Dense Fibrillary Component
Colocalizes With Gja1 At The Nuclear Membrane Specifically During Cell Cycle G1/S Phase
Alternative Antibody Names Anti-A-Kinase Anchor Protein 8 antibody
Anti-Akap-8 antibody
Anti-A-Kinase Anchor Protein 95 Kda antibody
Anti-Akap 95 antibody
Anti-AKAP8 antibody
Anti-AKAP95 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance