• Western blot analysis of extracts of various cell lines, using AGXT antibody (STJ110695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded human liver using AGXT Rabbit polyclonal antibody (STJ110695) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver using AGXT rabbit polyclonal antibody (STJ110695) at dilution of 1:150 (40x lens).
  • Western blot analysis of Mouse liver, using AGXT Rabbit polyclonal antibody (STJ110695) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AGXT antibody (183-392) (STJ110695)

SKU:
STJ110695

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AGXT (183-392) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AGXT
Gene ID: 189
Uniprot ID: AGT1_HUMAN
Immunogen Region: 183-392
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 183-392 of human AGXT (NP_000021.1).
Immunogen Sequence: DSVASLGGTPLYMDRQGIDI LYSGSQKALNAPPGTSLISF SDKAKKKMYSRKTKPFSFYL DIKWLANFWGCDDQPRMYHH TIPVISLYSLRESLALIAEQ GLENSWRQHREAAAYLHGRL QALGLQLFVKDPALRLPTVT TVAVPAGYDWRDIVSYVIDH FDIEIMGGLGPSTGKVLRIG LLGCNATRENVDRVTEALRA ALQHCPKKKL
Tissue Specificity Liver.
Function Peroxisomal aminotransferase that catalyzes the transamination of glyoxylate to glycine and contributes to the glyoxylate detoxification. Also catalyzes the transamination between L-serine and pyruvate and contributes to gluconeogenesis from the L-serine metabolism.
Protein Name Alanine--Glyoxylate Aminotransferase
Agt
Serine--Pyruvate Aminotransferase
Spt
Database Links Reactome: R-HSA-389661
Reactome: R-HSA-9033241
Cellular Localisation Peroxisome
Alternative Antibody Names Anti-Alanine--Glyoxylate Aminotransferase antibody
Anti-Agt antibody
Anti-Serine--Pyruvate Aminotransferase antibody
Anti-Spt antibody
Anti-AGXT antibody
Anti-AGT1 antibody
Anti-SPAT antibody

Information sourced from Uniprot.org

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