• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g AGPAT1 antibody (STJ28600). Western blot was performed from the immunoprecipitate using AGPAT1 antibody (STJ28600) at a dilution of 1:1000.
  • Western blot analysis of various lysates using AGPAT1 Rabbit polyclonal antibody (STJ28600) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AGPAT1 antibody (204-283) (STJ28600)

SKU:
STJ28600

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AGPAT1
Gene ID: 10554
Uniprot ID: PLCA_HUMAN
Immunogen Region: 204-283
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 204-283 of human AGPAT1 (NP_006402.1).
Immunogen Sequence: PIVPIVMSSYQDFYCKKERR FTSGQCQVRVLPPVPTEGLT PDDVPALADRVRHSMLTVFR EISTDGRGGGDYLKKPGGGG
Tissue Specificity Widely expressed. Expressed in adipose tissue and at high levels in testis and pancreas. Expressed at lower levels in tissues such as heart, brain, placenta, kidney, lung, spleen, thymus, prostate, ovary, intestine, colon, leukocyte and liver.
Function Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
Protein Name 1-Acyl-Sn-Glycerol-3-Phosphate Acyltransferase Alpha
1-Acylglycerol-3-Phosphate O-Acyltransferase 1
1-Agp Acyltransferase 1
1-Agpat 1
Lysophosphatidic Acid Acyltransferase Alpha
Lpaat-Alpha
Protein G15
Database Links Reactome: R-HSA-1483166
Reactome: R-HSA-163765
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-1-Acyl-Sn-Glycerol-3-Phosphate Acyltransferase Alpha antibody
Anti-1-Acylglycerol-3-Phosphate O-Acyltransferase 1 antibody
Anti-1-Agp Acyltransferase 1 antibody
Anti-1-Agpat 1 antibody
Anti-Lysophosphatidic Acid Acyltransferase Alpha antibody
Anti-Lpaat-Alpha antibody
Anti-Protein G15 antibody
Anti-AGPAT1 antibody
Anti-G15 antibody

Information sourced from Uniprot.org

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