• Immunofluorescence analysis of HepG2 cells (upper left) and LO2 cells (negative sample control) (upper right) using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP) ) Rabbit polyclonal antibody (red, A17457) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells (upper left) and LO2 cells (negative sample control) (upper right) using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP) ) Rabbit polyclonal antibody (red, STJ113691) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of mouse liver, using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP) ) antibody (STJ113691) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-AFP antibody (450-550) (STJ113691)

SKU:
STJ113691

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AFP (450-550) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AFP
Gene ID: 174
Uniprot ID: FETA_HUMAN
Immunogen Region: 450-550
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 450-550 of Alpha-Fetoprotein (AFP) (NP_001125.1).
Immunogen Sequence: ITRKMAATAATCCQLSEDKL LACGEGAADIIIGHLCIRHE MTPVNPGVGQCCTSSYANRR PCFSSLVVDETYVPPAFSDD KFIFHKDLCQAQGVALQTMK Q
Tissue Specificity Plasma. Synthesized by the fetal liver and yolk sac.
Post Translational Modifications Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated.
Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Protein Name Alpha-Fetoprotein
Alpha-1-Fetoprotein
Alpha-Fetoglobulin
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative Antibody Names Anti-Alpha-Fetoprotein antibody
Anti-Alpha-1-Fetoprotein antibody
Anti-Alpha-Fetoglobulin antibody
Anti-AFP antibody
Anti-HPAFP antibody

Information sourced from Uniprot.org

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