• Immunofluorescence analysis of U-2 OS cells using AEBP1 Rabbit polyclonal antibody (STJ118768) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using AEBP1 Rabbit polyclonal antibody (STJ118768) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using AEBP1 Rabbit polyclonal antibody (STJ118768) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse brain, using AEBP1 Rabbit polyclonal antibody (STJ118768) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-AEBP1 antibody (999-1158) (STJ118768)

SKU:
STJ118768

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AEBP1 (999-1158) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AEBP1
Gene ID: 165
Uniprot ID: AEBP1_HUMAN
Immunogen Region: 999-1158
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 999-1158 of human AEBP1 (NP_001120.3).
Immunogen Sequence: NRPIPHIDPSRPMTPQQRRL QQRRLQHRLRLRAQMRLRRL NATTTLGPHTVPPTLPPAPA TTLSTTIEPWGLIPPTTAGW EESETETYTEVVTEFGTEVE PEFGTKVEPEFETQLEPEFE TQLEPEFEEEEEEEKEEEIA TGQAFPFTTVETYTVNFGDF
Tissue Specificity Expressed in osteoblast and visceral fat.
Post Translational Modifications Phosphorylated by MAPK1 in vitro.
Function Isoform 1: As a positive regulator of collagen fibrillogenesis, it is probably involved in the organization and remodeling of the extracellular matrix. Isoform 2: May positively regulate MAP-kinase activity in adipocytes, leading to enhanced adipocyte proliferation and reduced adipocyte differentiation. May also positively regulate NF-kappa-B activity in macrophages by promoting the phosphorylation and subsequent degradation of I-kappa-B-alpha (NFKBIA), leading to enhanced macrophage inflammatory responsiveness. Can act as a transcriptional repressor.
Protein Name Adipocyte Enhancer-Binding Protein 1
Ae-Binding Protein 1
Aortic Carboxypeptidase-Like Protein
Cellular Localisation Isoform 1: Secreted
Isoform 2: Cytoplasm
Nucleus
Alternative Antibody Names Anti-Adipocyte Enhancer-Binding Protein 1 antibody
Anti-Ae-Binding Protein 1 antibody
Anti-Aortic Carboxypeptidase-Like Protein antibody
Anti-AEBP1 antibody
Anti-ACLP antibody

Information sourced from Uniprot.org

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