| Host: | Mouse |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Adiponectin (C-Term) is suitable for use in Western Blot research applications. |
| Clonality : | Monoclonal |
| Clone ID : | M576 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Protein G Purified |
| Dilution Range: | WB 1:500 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | Adipoq |
| Gene ID: | 11450 |
| Uniprot ID: | ADIPO_MOUSE |
| Immunogen Region: | C-Term |
| Specificity: | This antibody detects a 30 kDa* protein on SDS-PAGE immunoblots of human and mouse serum, as well as detects full length human and mouse recombinant proteins. |
| Immunogen: | Clone (M576) was generated from a recombinant protein that included amino acid residues within the C-terminal region of mouse Adiponectin. This sequence has high homology with similar regions in rat and human adiponectin. |
| Tissue Specificity | Synthesized exclusively by adipocytes and secreted into plasma. |
| Post Translational Modifications | HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagen-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes. O-glycosylated. Not N-glycosylated O-linked glycans on hydroxylysine residues consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. O-linked glycosylation in the N-terminal is disialylated with the structure Neu5Acalpha2->8Neu5Acalpha2->3Gal. Sialylated by alpha 2,8-sialyltransferase III. Succination of Cys-39 by the Krebs cycle intermediate fumarate, which leads to S-(2-succinyl)cysteine residues, inhibits polymerization and secretion of adiponectin. Adiponectin is a major target for succination in both adipocytes and adipose tissue of diabetic mice. It was proposed that succination of proteins is a biomarker of mitochondrial stress and accumulation of Krebs cycle intermediates in adipose tissue in diabetes and that succination of adiponectin may contribute to the decrease in plasma adiponectin in diabetes. |
| Function | Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. |
| Protein Name | Adiponectin 30 Kda Adipocyte Complement-Related Protein Adipocyte Complement-Related 30 Kda Protein Acrp30 Adipocyte - C1q And Collagen Domain-Containing Protein Adipocyte-Specific Protein Adipoq |
| Database Links | Reactome: R-MMU-163680 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Adiponectin antibody Anti-30 Kda Adipocyte Complement-Related Protein antibody Anti-Adipocyte Complement-Related 30 Kda Protein antibody Anti-Acrp30 antibody Anti-Adipocyte - C1q And Collagen Domain-Containing Protein antibody Anti-Adipocyte-Specific Protein Adipoq antibody Anti-Adipoq antibody Anti-Acdc antibody Anti-Acrp30 antibody Anti-Apm1 antibody |
Information sourced from Uniprot.org