| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ADHFE1 |
| Gene ID: | 137872 |
| Uniprot ID: | HOT_HUMAN |
| Immunogen Region: | 41-255 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 41-255 of human ADHFE1 (NP_653251.2). |
| Immunogen Sequence: | KTTDYAFEMAVSNIRYGAAV TKEVGMDLKNMGAKNVCLMT DKNLSKLPPVQVAMDSLVKN GIPFTVYDNVRVEPTDSSFM EAIEFAQKGAFDAYVAVGGG STMDTCKAANLYASSPHSDF LDYVSAPIGKGKPVSVPLKP LIAVPTTSGTGSETTGVAIF DYEHLKVKIGITSRAIKPTL GLIDPLHTLHMPARVVANSG FDVLCHALESYTTLP |
| Tissue Specificity | Only expressed in adult liver. |
| Function | Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). D,L-3-hydroxyisobutyrate and L-3-hydroxybutyrate (L-3-OHB) are also substrates for HOT with 10-fold lower activities. |
| Protein Name | Hydroxyacid-Oxoacid Transhydrogenase - MitochondrialHotAlcohol Dehydrogenase Iron-Containing Protein 1Adhfe1Fe-Containing Alcohol Dehydrogenase |
| Database Links | Reactome: R-HSA-880009 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Hydroxyacid-Oxoacid Transhydrogenase - Mitochondrial antibodyAnti-Hot antibodyAnti-Alcohol Dehydrogenase Iron-Containing Protein 1 antibodyAnti-Adhfe1 antibodyAnti-Fe-Containing Alcohol Dehydrogenase antibodyAnti-ADHFE1 antibodyAnti-HMFT2263 antibody |
Information sourced from Uniprot.org