• Western blot analysis of lysates from Mouse heart using ADHFE1 Rabbit polyclonal antibody (STJ11105629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ADHFE1 antibody (41-255) (STJ11105629)

SKU:
STJ11105629

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Hydroxyacid-Oxoacid Transhydrogenase-Mitochondrial (41-255) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADHFE1
Gene ID: 137872
Uniprot ID: HOT_HUMAN
Immunogen Region: 41-255
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 41-255 of human ADHFE1 (NP_653251.2).
Immunogen Sequence: KTTDYAFEMAVSNIRYGAAV TKEVGMDLKNMGAKNVCLMT DKNLSKLPPVQVAMDSLVKN GIPFTVYDNVRVEPTDSSFM EAIEFAQKGAFDAYVAVGGG STMDTCKAANLYASSPHSDF LDYVSAPIGKGKPVSVPLKP LIAVPTTSGTGSETTGVAIF DYEHLKVKIGITSRAIKPTL GLIDPLHTLHMPARVVANSG FDVLCHALESYTTLP
Tissue Specificity Only expressed in adult liver.
Function Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). D,L-3-hydroxyisobutyrate and L-3-hydroxybutyrate (L-3-OHB) are also substrates for HOT with 10-fold lower activities.
Protein Name Hydroxyacid-Oxoacid Transhydrogenase - Mitochondrial
Hot
Alcohol Dehydrogenase Iron-Containing Protein 1
Adhfe1
Fe-Containing Alcohol Dehydrogenase
Database Links Reactome: R-HSA-880009
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Hydroxyacid-Oxoacid Transhydrogenase - Mitochondrial antibody
Anti-Hot antibody
Anti-Alcohol Dehydrogenase Iron-Containing Protein 1 antibody
Anti-Adhfe1 antibody
Anti-Fe-Containing Alcohol Dehydrogenase antibody
Anti-ADHFE1 antibody
Anti-HMFT2263 antibody

Information sourced from Uniprot.org

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