• Immunofluorescence analysis of Hep G2 cells using ADH5/GSNOR Rabbit polyclonal antibody (STJ22518) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ADH5/GSNOR Rabbit polyclonal antibody (STJ22518) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using ADH5/GSNOR Rabbit polyclonal antibody (STJ22518) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of SH-SY5Y cells using ADH5/GSNOR Rabbit polyclonal antibody (STJ22518) at a dilution of 1:50 (40x lens). Secondary antibody: () at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ADH5/GSNOR Rabbit polyclonal antibody (STJ22518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-ADH5 antibody (1-374) (STJ22518)

SKU:
STJ22518

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADH5 (1-374) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADH5
Gene ID: 128
Uniprot ID: ADHX_HUMAN
Immunogen Region: 1-374
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-374 of human ADH5/GSNOR (NP_000662.3).
Immunogen Sequence: MANEVIKCKAAVAWEAGKPL SIEEIEVAPPKAHEVRIKII ATAVCHTDAYTLSGADPEGC FPVILGHEGAGIVESVGEGV TKLKAGDTVIPLYIPQCGEC KFCLNPKTNLCQKIRVTQGK GLMPDGTSRFTCKGKTILHY MGTSTFSEYTVVADISVAKI DPLAPLDKVCLLGCGISTGY GAAVNTAKLEPGSVCAVFGL GGVGLAVIMGCKVAGASRII GVDINKDKFARAKEFGATE
Function Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Class-III ADH is remarkably ineffective in oxidizing ethanol. Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage.
Protein Name Alcohol Dehydrogenase Class-3
Alcohol Dehydrogenase 5
Alcohol Dehydrogenase Class Chi Chain
Alcohol Dehydrogenase Class-Iii
Glutathione-Dependent Formaldehyde Dehydrogenase
Faldh
Fdh
Gsh-Fdh
S-(Hydroxymethylglutathione Dehydrogenase
Database Links Reactome: R-HSA-71384
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Alcohol Dehydrogenase Class-3 antibody
Anti-Alcohol Dehydrogenase 5 antibody
Anti-Alcohol Dehydrogenase Class Chi Chain antibody
Anti-Alcohol Dehydrogenase Class-Iii antibody
Anti-Glutathione-Dependent Formaldehyde Dehydrogenase antibody
Anti-Faldh antibody
Anti-Fdh antibody
Anti-Gsh-Fdh antibody
Anti-S-(Hydroxymethylglutathione Dehydrogenase antibody
Anti-ADH5 antibody
Anti-ADHX antibody
Anti-FDH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance