• Western blot analysis of extracts of various cell lines, using ADAMTSL1 antibody (STJ110376) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Western blot analysis of extracts of various cell lines, using ADAMTSL1 antibody (STJ110376) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-ADAMTSL1 antibody (1483-1762) (STJ110376)

SKU:
STJ110376

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADAMTSL1 (1483-1762) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADAMTSL1
Gene ID: 92949
Uniprot ID: ATL1_HUMAN
Immunogen Region: 1483-1762
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1483-1762 of human ADAMTSL1 (NP_001035362.3).
Immunogen Sequence: SLVIQDYWWSVDRLATCSAS CGNRGVQQPRLRCLLNSTEV NPAHCAGKVRPAVQPIACNR RDCPSRWMVTSWSACTRSCG GGVQTRRVTCQKLKASGIST PVSNDMCTQVAKRPVDTQAC NQQLCVEWAFSSWGQCNGPC IGPHLAVQHRQVFCQTRDGI TLPSEQCSALPRPVSTQNCW SEACSVHWRVSLWTLCTATC GNYGFQSRRVECVHARTNKA VPEHLCSWGPRPANWQRCN
Tissue Specificity Expressed primarily in adult skeletal muscle.
Post Translational Modifications C-, N- and O-glycosylated. O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTSL1. Can also be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion. Disulfide bonds are present.
Protein Name Adamts-Like Protein 1
Adamtsl-1
Punctin-1
Database Links Reactome: R-HSA-5083635
Reactome: R-HSA-5173214
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Adamts-Like Protein 1 antibody
Anti-Adamtsl-1 antibody
Anti-Punctin-1 antibody
Anti-ADAMTSL1 antibody
Anti-ADAMTSR1 antibody
Anti-C9orf94 antibody
Anti-UNQ528 antibody
Anti-PRO1071 antibody

Information sourced from Uniprot.org

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