• Immunofluorescence analysis of PC-12 cells using ADAM9 Rabbit polyclonal antibody (STJ27341) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ADAM9 Rabbit polyclonal antibody (STJ27341) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ADAM9 Rabbit polyclonal antibody (STJ27341) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ADAM9 antibody (29-210) (STJ27341)

SKU:
STJ27341

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADAM9 (29-210) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADAM9
Gene ID: 8754
Uniprot ID: ADAM9_HUMAN
Immunogen Region: 29-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-210 of human ADAM9 (NP_003807.1).
Immunogen Sequence: AARPGFQQTSHLSSYEIITP WRLTRERREAPRPYSKQVSY VIQAEGKEHIIHLERNKDLL PEDFVVYTYNKEGTLITDHP NIQNHCHYRGYVEGVHNSSI ALSDCFGLRGLLHLENASYG IEPLQNSSHFEHIIYRMDDV YKEPLKCGVSNKDIEKETAK DEEEEPPSMTQLLRRRRAVL PQ
Tissue Specificity Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart.
Post Translational Modifications Proteolytically cleaved in the trans-Golgi network before it reaches the plasma membrane to generate a mature protein. The removal of the pro-domain occurs via cleavage at two different sites. Processed most likely by a pro-protein convertase such as furin, at the boundary between the pro-domain and the catalytic domain. An additional upstream cleavage pro-protein convertase site (Arg-56/Glu-57) has an important role in the activation of ADAM9. Phosphorylation is induced in vitro by phorbol-12-myristate-13-acetate (PMA).
Function Metalloprotease that cleaves and releases a number of molecules with important roles in tumorigenesis and angiogenesis, such as TEK, KDR, EPHB4, CD40, VCAM1 and CDH5. May mediate cell-cell, cell-matrix interactions and regulate the motility of cells via interactions with integrins. Isoform 2: May act as alpha-secretase for amyloid precursor protein (APP).
Protein Name Disintegrin And Metalloproteinase Domain-Containing Protein 9
Adam 9
Cellular Disintegrin-Related Protein
Meltrin-Gamma
Metalloprotease/Disintegrin/Cysteine-Rich Protein 9
Myeloma Cell Metalloproteinase
Database Links Reactome: R-HSA-1442490
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Secreted
Alternative Antibody Names Anti-Disintegrin And Metalloproteinase Domain-Containing Protein 9 antibody
Anti-Adam 9 antibody
Anti-Cellular Disintegrin-Related Protein antibody
Anti-Meltrin-Gamma antibody
Anti-Metalloprotease/Disintegrin/Cysteine-Rich Protein 9 antibody
Anti-Myeloma Cell Metalloproteinase antibody
Anti-ADAM9 antibody
Anti-KIAA0021 antibody
Anti-MCMP antibody
Anti-MDC9 antibody
Anti-MLTNG antibody

Information sourced from Uniprot.org

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