• Immunofluorescence analysis of NIH/3T3 cells using ADAM11 Rabbit polyclonal antibody (STJ116462) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ADAM11 Rabbit polyclonal antibody (STJ116462) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ADAM11 Rabbit polyclonal antibody (STJ116462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-ADAM11 antibody (290-430) (STJ116462)

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STJ116462

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADAM11 (290-430) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADAM11
Gene ID: 4185
Uniprot ID: ADA11_HUMAN
Immunogen Region: 290-430
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 290-430 of human ADAM11 (NP_002381.2).
Immunogen Sequence: METWADGDKIQVQDDLLETL ARLMVYRREGLPEPSDATHL FSGRTFQSTSSGAAYVGGIC SLSHGGGVNEYGNMGAMAVT LAQTLGQNLGMMWNKHRSSA GDCKCPDIWLGCIMEDTGFY LPRKFSRCSIDEYNQFLQEG G
Tissue Specificity Expressed predominantly in brain. Slightly detected or not at all in other tissues.
Post Translational Modifications The precursor is cleaved by a furin endopeptidase.
Function Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Required for localization of the potassium channel subunit proteins KCNA1/KV1.1 and KCNA2/KV1.2 at cerebellar cortex basket cell distal terminals, is thereby involved in ephaptic inhibitory synchronization of Purkinje cell firing and response to stress. Plays a role in spatial learning and motor coordination. Involved in the nociceptive pain response to chemical-derived stimulation.
Protein Name Disintegrin And Metalloproteinase Domain-Containing Protein 11
Adam 11
Metalloproteinase-Like - Disintegrin-Like - And Cysteine-Rich Protein
Mdc
Database Links Reactome: R-HSA-5682910
Cellular Localisation Presynaptic Cell Membrane
Single-Pass Type I Membrane Protein
Perikaryon
Cell Projection
Axon
Localizes To Basket Cell Terminals And Pinceaux
Alternative Antibody Names Anti-Disintegrin And Metalloproteinase Domain-Containing Protein 11 antibody
Anti-Adam 11 antibody
Anti-Metalloproteinase-Like - Disintegrin-Like - And Cysteine-Rich Protein antibody
Anti-Mdc antibody
Anti-ADAM11 antibody
Anti-MDC antibody

Information sourced from Uniprot.org

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