Anti-ACPP/PAP antibody (269-282) (STJ72574)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ACPP/PAP (269-282) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 50kDa band observed in Human Prostate lysates (calculated MW of 48.3kDa according to NP_001127666.1). Recommended concentration: 0.01-0.03ยตg/ml.IHC: In paraffin embedded Hu |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ACP3 |
| Gene ID | 55 |
| Uniprot ID | PPAP_HUMAN |
| Accession Number | NP_001127666.1; NP_001090.2 |
| Immunogen Region | 269-282 |
| Immunogen Sequence | NHMKRATQIPSYKK |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_001127666.1; NP_001090.2). |
Additional Info
| Post Translational Modifications | N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides. Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection). |
| Function | A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Isoform 2: Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling. In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor. PAPf39: (Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea. Target cell attachment and enhancement of HIV infection is inhibited by surfen. Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection. |
| Protein Name | Prostatic Acid PhosphatasePap5'-Nucleotidase5'-NtAcid Phosphatase 3Ecto-5'-NucleotidaseProtein Tyrosine Phosphatase Acp3Thiamine MonophosphataseTmpase Cleaved Into - Papf39 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform 1: SecretedIsoform 2: Cell MembraneSingle-Pass Type I Membrane ProteinLysosome MembraneNucleusCytoplasmCytosolAppears To Shuttle Between The Cell Membrane And Intracellular VesiclesColocalizes With Flot1 At Cell Membrane And In Intracellular VesiclesColocalizes With Lamp2 On The Lysosome Membrane |
| Alternative Antibody Names | Anti-Prostatic Acid Phosphatase antibodyAnti-Pap antibodyAnti-5'-Nucleotidase antibodyAnti-5'-Nt antibodyAnti-Acid Phosphatase 3 antibodyAnti-Ecto-5'-Nucleotidase antibodyAnti-Protein Tyrosine Phosphatase Acp3 antibodyAnti-Thiamine Monophosphatase antibodyAnti-Tmpase Cleaved Into - Papf39 antibodyAnti-ACP3 antibodyAnti-ACPP antibody |
Information sourced from Uniprot.org