• Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Acetyl-KPNA2-Lys22 antibody (STJA0005974)

SKU:
STJA0005974

€57.50 - €282.50
Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Acetyl-Importin subunit alpha-1-Lys22 is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-p 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KPNA2
Gene ID: 3838
Uniprot ID: IMA1_HUMAN
Specificity: This antibody detects endogenous levels of Human, Mouse, Rat Karyopherin Alpha 2 (Acetyl Lys22)
Immunogen: Synthesized peptide derived from human Karyopherin Alpha 2 (Acetyl Lys22)
Tissue Specificity Expressed ubiquitously.
Function Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
Protein Name Importin Subunit Alpha-1
Karyopherin Subunit Alpha-2
Rag Cohort Protein 1
Srp1-Alpha
Database Links Reactome: R-HSA-111932
Reactome: R-HSA-1169408
Reactome: R-HSA-168276
Reactome: R-HSA-442729
Reactome: R-HSA-5693548
Reactome: R-HSA-9018519
Reactome: R-HSA-9692916
Reactome: R-HSA-9705671
Cellular Localisation Cytoplasm
Nucleus
Endoplasmic Reticulum Membrane
Golgi Apparatus Membrane
(Microbial Infection) Retained In Er/Golgi Membranes Upon Interaction With Sars-Cov Virus Orf6 Protein
Alternative Antibody Names Anti-Importin Subunit Alpha-1 antibody
Anti-Karyopherin Subunit Alpha-2 antibody
Anti-Rag Cohort Protein 1 antibody
Anti-Srp1-Alpha antibody
Anti-KPNA2 antibody
Anti-RCH1 antibody
Anti-SRP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance