• Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K15 Rabbit polyclonal antibody (STJ118070) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K15 Rabbit polyclonal antibody (STJ118070) at dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K15 Rabbit polyclonal antibody (STJ118070) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Acetyl-Histone H2B-K15 in paraffin-embedded mouse brain using Acetyl-Histone H2B-K15 Rabbit polyclonal antibody (STJ118070) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Acetyl-Histone H2B-K15 in paraffin-embedded human gastric cancer using Acetyl-Histone H2B-K15 Rabbit polyclonal antibody (STJ118070) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Acetyl-Histone H2B-K15 in paraffin-embedded human breast cancer using Acetyl-Histone H2B-K15 Rabbit polyclonal antibody (STJ118070) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Acetyl-Histone H2B-K15 in paraffin-embedded rat lung using Acetyl-Histone H2B-K15 Rabbit polyclonal antibody (STJ118070) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Acetyl-Histone H2B-K15 Rabbit polyclonal antibody (STJ118070) at 1:1000 dilution. HeLa cells were treated by TSA (1 uM) at 37 °C for 18 hours. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Acetyl-Histone H2B-K15 antibody (STJ118070)

SKU:
STJ118070

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Acetyl-Histone H2B-K15 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic acetylated peptide around K15 of human Histone H2B (NP_003519.1).
Immunogen Sequence: AQKKD
Background Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the engrailed (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

Information sourced from Uniprot.org

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