| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Acetyl-Erythroid transcription factor-Lys312 is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:1000-2000ELISA 1:5000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GATA1 |
| Gene ID: |
2623 |
| Uniprot ID: |
GATA1_HUMAN |
| Specificity: |
This antibody detects endogenous levels of Human, Mouse, Rat GATA-1 (Acetyl Lys312) |
| Immunogen: |
Synthesized peptide derived from human GATA-1 (Acetyl Lys312) |
| Tissue Specificity | Erythrocytes. |
| Post Translational Modifications | Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137. Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity. Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo. Acetylated on Lys-233, Lys-245 Lys-246 by EP300. |
| Function | Transcriptional activator or repressor which serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-ATGATAAG-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS. |
| Protein Name | Erythroid Transcription FactorEryf1Gata-Binding Factor 1Gata-1Gf-1Nf-E1 Dna-Binding Protein |
| Database Links | Reactome: R-HSA-8936459Reactome: R-HSA-8939236Reactome: R-HSA-983231 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Erythroid Transcription Factor antibodyAnti-Eryf1 antibodyAnti-Gata-Binding Factor 1 antibodyAnti-Gata-1 antibodyAnti-Gf-1 antibodyAnti-Nf-E1 Dna-Binding Protein antibodyAnti-GATA1 antibodyAnti-ERYF1 antibodyAnti-GF1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
