| Host: | Goat |
| Applications: | Pep-ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-ACACB (146-159) is suitable for use in ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: Not yet tested-our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 277kDa according to NP_001084.3. Therefore we canno |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | ACACB |
| Gene ID: | 32 |
| Uniprot ID: | ACACB_HUMAN |
| Immunogen Region: | 146-159 |
| Accession Number: | NP_001084.3 |
| Immunogen Sequence: | SPSKEDKKQANIKR |
| Post Translational Modifications | The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity. Phosphorylation at Ser-222 by AMPK inactivates the enzyme. Required for the maintenance of skeletal muscle lipid and glucose homeostasis. |
| Function | Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism. Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA. Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation. Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage. |
| Protein Name | Acetyl-Coa Carboxylase 2 Acc-Beta |
| Database Links | Reactome: R-HSA-163765 Reactome: R-HSA-196780 Reactome: R-HSA-200425 Reactome: R-HSA-2426168 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Acetyl-Coa Carboxylase 2 antibody Anti-Acc-Beta antibody Anti-ACACB antibody Anti-ACC2 antibody Anti-ACCB antibody |
Information sourced from Uniprot.org