Anti-ACACB antibody (146-159) (STJ72668)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ACACB (146-159) is suitable for use in ELISA research applications. |
| Applications | Pep-ELISA |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Not yet tested-our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 277kDa according to NP_001084.3. Therefore we canno |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ACACB |
| Gene ID | 32 |
| Uniprot ID | ACACB_HUMAN |
| Accession Number | NP_001084.3 |
| Immunogen Region | 146-159 |
| Immunogen Sequence | SPSKEDKKQANIKR |
Additional Info
| Post Translational Modifications | The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity. Phosphorylation at Ser-222 by AMPK inactivates the enzyme. Required for the maintenance of skeletal muscle lipid and glucose homeostasis. |
| Function | Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism. Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA. Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation. Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage. |
| Protein Name | Acetyl-Coa Carboxylase 2Acc-Beta |
| Database Links | Reactome: R-HSA-163765Reactome: R-HSA-196780Reactome: R-HSA-200425Reactome: R-HSA-2426168 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Acetyl-Coa Carboxylase 2 antibodyAnti-Acc-Beta antibodyAnti-ACACB antibodyAnti-ACC2 antibodyAnti-ACCB antibody |
Information sourced from Uniprot.org