• Western blot analysis of extracts of K-562 cells, using BGAT antibody (STJ22473) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-ABO antibody (54-354) (STJ22473)

SKU:
STJ22473

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ABO (54-354) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABO
Gene ID: 28
Uniprot ID: BGAT_HUMAN
Immunogen Region: 54-354
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 54-354 of human BGAT (NP_065202.2).
Immunogen Sequence: AVREPDHLQRVSLPRMVYPQ PKVLTPCRKDVLVVTPWLAP IVWEGTFNIDILNEQFRLQN TTIGLTVFAIKKYVAFLKLF LETAEKHFMVGHRVHYYVFT DQPAAVPRVTLGTGRQLSVL EVRAYKRWQDVSMRRMEMIS DFCERRFLSEVDYLVCVDVD MEFRDHVGVEILTPLFGTLH PGFYGSSREAFTYERRPQSQ AYIPKDEGDFYYLGGFFGGS VQEVQRLTRACHQAMMVDQ
Post Translational Modifications The soluble form derives from the membrane form by proteolytic processing.
Function This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.
Protein Name Histo-Blood Group Abo System Transferase
Fucosylglycoprotein 3-Alpha-Galactosyltransferase
Fucosylglycoprotein Alpha-N-Acetylgalactosaminyltransferase
Glycoprotein-Fucosylgalactoside Alpha-N-Acetylgalactosaminyltransferase
Glycoprotein-Fucosylgalactoside Alpha-Galactosyltransferase
Histo-Blood Group A Transferase
A Transferase
Histo-Blood Group B Transferase
B Transferase
Nagat Cleaved Into - Fucosylglycoprotein Alpha-N-Acetylgalactosaminyltransferase Soluble Form
Database Links Reactome: R-HSA-9033807
Cellular Localisation Golgi Apparatus
Golgi Stack Membrane
Single-Pass Type Ii Membrane Protein
Secreted
Membrane-Bound Form In Trans Cisternae Of Golgi
Secreted Into The Body Fluid
Alternative Antibody Names Anti-Histo-Blood Group Abo System Transferase antibody
Anti-Fucosylglycoprotein 3-Alpha-Galactosyltransferase antibody
Anti-Fucosylglycoprotein Alpha-N-Acetylgalactosaminyltransferase antibody
Anti-Glycoprotein-Fucosylgalactoside Alpha-N-Acetylgalactosaminyltransferase antibody
Anti-Glycoprotein-Fucosylgalactoside Alpha-Galactosyltransferase antibody
Anti-Histo-Blood Group A Transferase antibody
Anti-A Transferase antibody
Anti-Histo-Blood Group B Transferase antibody
Anti-B Transferase antibody
Anti-Nagat Cleaved Into - Fucosylglycoprotein Alpha-N-Acetylgalactosaminyltransferase Soluble Form antibody
Anti-ABO antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance