• Immunofluorescence analysis of L929 cells using ABHD4 Rabbit polyclonal antibody (STJ11102928) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Rat testis, using ABHD4 Rabbit polyclonal antibody (STJ11102928) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ABHD4 antibody (223-342) (STJ11102928)

SKU:
STJ11102928

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ABHD4 (223-342) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABHD4
Gene ID: 63874
Uniprot ID: ABHD4_HUMAN
Immunogen Region: 223-342
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 223-342 of human ABHD4 (NP_071343.2).
Immunogen Sequence: PDFKRKFADFFEDDTISEYI YHCNAQNPSGETAFKAMMES FGWARRPMLERIHLIRKDVP ITMIYGSDTWIDTSTGKKVK MQRPDSYVRDMEIKGASHHV YADQPHIFNAVVEEICDSVD
Function Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine.
Protein Name (Lyso-N-Acylphosphatidylethanolamine Lipase
Alpha/Beta Hydrolase Domain-Containing Protein 4
Abhydrolase Domain-Containing Protein 4
Alpha/Beta-Hydrolase 4
Database Links Reactome: R-HSA-1482839
Alternative Antibody Names Anti-(Lyso-N-Acylphosphatidylethanolamine Lipase antibody
Anti-Alpha/Beta Hydrolase Domain-Containing Protein 4 antibody
Anti-Abhydrolase Domain-Containing Protein 4 antibody
Anti-Alpha/Beta-Hydrolase 4 antibody
Anti-ABHD4 antibody

Information sourced from Uniprot.org

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