• Immunofluorescence analysis of HeLa cells using ABCB8 Polyclonal Antibody (STJ22457) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using ABCB8 antibody (STJ22457) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using ABCB8 antibody (STJ22457) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver damage using ABCB8 antibody (STJ22457) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ABCB8 antibody (STJ22457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ABCB8 antibody (469-718) (STJ22457)

SKU:
STJ22457

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:100
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABCB8
Gene ID: 11194
Uniprot ID: MITOS_HUMAN
Immunogen Region: 469-718
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 469-718 of human ABCB8 (NP_009119.2).
Immunogen Sequence: GFEVLKDFTLTLPPGKIVAL VGQSGGGKTTVASLLERFYD PTAGVVMLDGRDLRTLDPSW LRGQVVGFISQEPVLFGTTI MENIRFGKLEASDEEVYTAA REANAHEFITSFPEGYNTVV GERGTTLSGGQKQRLAIARA LIKQPTVLILDEATSALDAE SERVVQEALDRASAGRTVLV IAHRLSTVRGAHCIVVMADG RVWEAGTHEELLKKGGLYAE LIRRQALDAPRTAAPPPKK
Tissue Specificity Ubiquitous.
Function ATP-binding subunit of the mitochondrial ATP-gated potassium channel (mitoK(ATP)). Together with pore-forming subunit CCDC51/MITOK of the mitoK(ATP) channel, mediates ATP-dependent potassium currents across the mitochondrial inner membrane. An increase in ATP intracellular levels closes the channel, inhibiting K(+) transport, whereas a decrease in ATP levels enhances K(+) uptake in the mitochondrial matrix. Plays a role in mitochondrial iron transport. Required for maintenance of normal cardiac function, possibly by influencing mitochondrial iron export and regulating the maturation of cytosolic iron sulfur cluster-containing enzymes.
Protein Name Mitochondrial Potassium Channel Atp-Binding Subunit
Atp-Binding Cassette Sub-Family B Member 8 - Mitochondrial
Abcb8
Mitochondrial Atp-Binding Cassette 1
M-Abc1
Mitochondrial Sulfonylurea-Receptor
Mitosur
Database Links Reactome: R-HSA-1369007
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Mitochondrial Potassium Channel Atp-Binding Subunit antibody
Anti-Atp-Binding Cassette Sub-Family B Member 8 - Mitochondrial antibody
Anti-Abcb8 antibody
Anti-Mitochondrial Atp-Binding Cassette 1 antibody
Anti-M-Abc1 antibody
Anti-Mitochondrial Sulfonylurea-Receptor antibody
Anti-Mitosur antibody
Anti-ABCB8 antibody
Anti-MABC1 antibody
Anti-MITOSUR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance