Anti-ABCB5 antibody (Internal) (STJ71387)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ71387-100
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General Information

Short DescriptionGoat polyclonal antibody anti-ABCB5 (Internal) is suitable for use in ELISA, Western Blot and Flow Cytometry research applications.
ApplicationsPep-ELISA/WB/FC
HostGoat
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx 80kDa band observed in lysates of cell line A431 (calculated MW of 89.8kDa according to NP_848654.3). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
F
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolABCB5
Gene ID340273
Uniprot IDABCB5_HUMAN
Accession NumberNP_848654.3; NP_001157413.1
Immunogen RegionInternal
Immunogen SequenceQTQHRNTSKKAQ
SpecificityThis antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1)

Additional Info

Function Energy-dependent efflux transporter responsible for decreased drug accumulation in multidrug-resistant cells. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair.
Protein Name Atp-Binding Cassette Sub-Family B Member 5
Abcb5 P-Gp
P-Glycoprotein Abcb5
Database Links Reactome: R-HSA-382556
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Atp-Binding Cassette Sub-Family B Member 5 antibody
Anti-Abcb5 P-Gp antibody
Anti-P-Glycoprotein Abcb5 antibody
Anti-ABCB5 antibody

Information sourced from Uniprot.org

Citations

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