| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ABCA3 (950-1050 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
ABCA3 |
| Gene ID: |
21 |
| Uniprot ID: |
ABCA3_HUMAN |
| Immunogen Region: |
950-1050 aa |
| Specificity: |
Specific for ABCA3. |
| Immunogen: |
A synthetic peptide from aa region 950-1050 of human ABCA3 conjugated to blue carrier protein was used as the antigen. |
| Post Translational Modifications | N-glycosylated. Localization at intracellular vesicles is accompanied by processing of oligosaccharide from high mannose type to complex type. N-linked glycosylation at Asn-124 and Asn-140 is required for stability and efficient anterograde trafficking and prevents from proteasomal degradation. Proteolytically cleaved by CTSL and to a lower extent by CTSB within multivesicular bodies (MVB) and lamellar bodies (LB) leading to a mature form of 150 kDa. |
| Function | Catalyzes the ATP-dependent transport of phospholipids such as phosphatidylcholine and phosphoglycerol from the cytoplasm into the lumen side of lamellar bodies, in turn participates in the lamellar bodies biogenesis and homeostasis of pulmonary surfactant. Transports preferentially phosphatidylcholine containing short acyl chains. In addition plays a role as an efflux transporter of miltefosine across macrophage membranes and free cholesterol (FC) through intralumenal vesicles by removing FC from the cell as a component of surfactant and protects cells from free cholesterol toxicity. |
| Protein Name | Phospholipid-Transporting Atpase Abca3Abc-C TransporterAtp-Binding Cassette Sub-Family A Member 3Atp-Binding Cassette Transporter 3Atp-Binding Cassette 3Xenobiotic-Transporting Atpase Abca3 Cleaved Into - 150 Kda Mature Form |
| Database Links | Reactome: R-HSA-1369062Reactome: R-HSA-5683678Reactome: R-HSA-5683826Reactome: R-HSA-5688399 |
| Cellular Localisation | EndosomeMultivesicular Body MembraneMulti-Pass Membrane ProteinCytoplasmic Vesicle MembraneLate Endosome MembraneLysosome MembraneLocalized In The Limiting Membrane Of Lamellar Bodies In Lung Alveolar Type Ii CellsTrafficks Via The GolgiSorting Vesicles (Svs) And Late Endosome/Multivesicular Body Network Directly To The Outer Membrane Of Lamellar Bodies In At2 Lung Epithelial Cells Or To Lysosomes And Lysosomal-Related Organelles (Lros) In Other Cells Where Undergoes Proteolytic Cleavage And Oligosaccharide Processing From High Mannose Type To Complex TypeOligomers Formation Takes Place In A Post-Endoplasmic Reticulum Compartment |
| Alternative Antibody Names | Anti-Phospholipid-Transporting Atpase Abca3 antibodyAnti-Abc-C Transporter antibodyAnti-Atp-Binding Cassette Sub-Family A Member 3 antibodyAnti-Atp-Binding Cassette Transporter 3 antibodyAnti-Atp-Binding Cassette 3 antibodyAnti-Xenobiotic-Transporting Atpase Abca3 Cleaved Into - 150 Kda Mature Form antibodyAnti-ABCA3 antibodyAnti-ABC3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
