• Western blot analysis of extracts of various cell lines, using TNRC6A antibody (STJ29936) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U2OS cells using TNRC6A antibody (STJ29936) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-TNRC6A antibody (950-1050) (STJ29936)

SKU:
STJ29936

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Host: Rabbit
Applications: WB, IF
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-TNRC6A (950-1050) is suitable for use in Western Blot and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF 1:50-1:100
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: TNRC6A
Gene ID: 27327
Uniprot ID: TNR6A_HUMAN
Immunogen Region: 950-1050
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 950-1050 of human TNRC6A (NP_055309.2).
Tissue Specificity Ubiquitous.
Function Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. As a scaffolding protein, associates with argonaute proteins bound to partially complementary mRNAs, and can simultaneously recruit CCR4-NOT and PAN deadenylase complexes.
Protein Name Trinucleotide Repeat-Containing Gene 6a Protein
Cag Repeat Protein 26
Emsy Interactor Protein
Gw182 Autoantigen
Protein Gw1
Glycine-Tryptophan Protein Of 182 Kda
Database Links Reactome: R-HSA-1912408
Reactome: R-HSA-2559580
Reactome: R-HSA-2559585
Reactome: R-HSA-4086398
Reactome: R-HSA-426496
Reactome: R-HSA-5578749
Reactome: R-HSA-5628897
Reactome: R-HSA-5687128
Reactome: R-HSA-8853884
Reactome: R-HSA-8934593
Reactome: R-HSA-8936459
Reactome: R-HSA-8943723
Reactome: R-HSA-8948700
Reactome: R-HSA-8986944
Reactome: R-HSA-9018519
Reactome: R-HSA-9022692
Reactome: R-HSA-9029569
Cellular Localisation Cytoplasm
P-Body
Mammalian P-Bodies Are Also Known As Gw Bodies (Gwbs)
Alternative Antibody Names Anti-Trinucleotide Repeat-Containing Gene 6a Protein antibody
Anti-Cag Repeat Protein 26 antibody
Anti-Emsy Interactor Protein antibody
Anti-Gw182 Autoantigen antibody
Anti-Protein Gw1 antibody
Anti-Glycine-Tryptophan Protein Of 182 Kda antibody
Anti-TNRC6A antibody
Anti-CAGH26 antibody
Anti-KIAA1460 antibody
Anti-TNRC6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance