| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ABCA3 (43-203) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ABCA3 |
| Gene ID: |
21 |
| Uniprot ID: |
ABCA3_HUMAN |
| Immunogen Region: |
43-203 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 43-203 of human ABCA3 (NP_001080.2). |
| Immunogen Sequence: |
RLKIQSENVPNATIYPGQSI QELPLFFTFPPPGDTWELAY IPSHSDAAKTVTETVRRALV INMRVRGFPSEKDFEDYIRY DNCSSSVLAAVVFEHPFNHS KEPLPLAVKYHLRFSYTRRN YMWTQTGSFFLKETEGWHTT SLFPLFPNPGPREPTSPDGG E |
| Tissue Specificity | Expressed in brain, pancreas, skeletal muscle and heart. Highly expressed in the lung in an AT2-cell-specific manner. Weakly expressed in placenta, kidney and liver. Also expressed in medullary thyroid carcinoma cells (MTC) and in C-cell carcinoma. |
| Post Translational Modifications | N-glycosylated. Localization at intracellular vesicles is accompanied by processing of oligosaccharide from high mannose type to complex type. N-linked glycosylation at Asn-124 and Asn-140 is required for stability and efficient anterograde trafficking and prevents from proteasomal degradation. Proteolytically cleaved by CTSL and to a lower extent by CTSB within multivesicular bodies (MVB) and lamellar bodies (LB) leading to a mature form of 150 kDa. |
| Function | Catalyzes the ATP-dependent transport of phospholipids such as phosphatidylcholine and phosphoglycerol from the cytoplasm into the lumen side of lamellar bodies, in turn participates in the lamellar bodies biogenesis and homeostasis of pulmonary surfactant. Transports preferentially phosphatidylcholine containing short acyl chains. In addition plays a role as an efflux transporter of miltefosine across macrophage membranes and free cholesterol (FC) through intralumenal vesicles by removing FC from the cell as a component of surfactant and protects cells from free cholesterol toxicity. |
| Protein Name | Phospholipid-Transporting Atpase Abca3Abc-C TransporterAtp-Binding Cassette Sub-Family A Member 3Atp-Binding Cassette Transporter 3Atp-Binding Cassette 3Xenobiotic-Transporting Atpase Abca3 Cleaved Into - 150 Kda Mature Form |
| Database Links | Reactome: R-HSA-1369062Reactome: R-HSA-5683678Reactome: R-HSA-5683826Reactome: R-HSA-5688399 |
| Cellular Localisation | EndosomeMultivesicular Body MembraneMulti-Pass Membrane ProteinCytoplasmic Vesicle MembraneLate Endosome MembraneLysosome MembraneLocalized In The Limiting Membrane Of Lamellar Bodies In Lung Alveolar Type Ii CellsTrafficks Via The GolgiSorting Vesicles (Svs) And Late Endosome/Multivesicular Body Network Directly To The Outer Membrane Of Lamellar Bodies In At2 Lung Epithelial Cells Or To Lysosomes And Lysosomal-Related Organelles (Lros) In Other Cells Where Undergoes Proteolytic Cleavage And Oligosaccharide Processing From High Mannose Type To Complex TypeOligomers Formation Takes Place In A Post-Endoplasmic Reticulum Compartment |
| Alternative Antibody Names | Anti-Phospholipid-Transporting Atpase Abca3 antibodyAnti-Abc-C Transporter antibodyAnti-Atp-Binding Cassette Sub-Family A Member 3 antibodyAnti-Atp-Binding Cassette Transporter 3 antibodyAnti-Atp-Binding Cassette 3 antibodyAnti-Xenobiotic-Transporting Atpase Abca3 Cleaved Into - 150 Kda Mature Form antibodyAnti-ABCA3 antibodyAnti-ABC3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
