| Host: |
Rabbit |
| Applications: |
IF |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Glucosylceramide Transporter Abca12 (2456-2595) is suitable for use in Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ABCA12 |
| Gene ID: |
26154 |
| Uniprot ID: |
ABCAC_HUMAN |
| Immunogen Region: |
2456-2595 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 2456-2595 of human ABCA12 (NP_775099.2). |
| Immunogen Sequence: |
CTRLAIMVNGKFQCIGSLQH IKSRFGRGFTVKVHLKNNKV TMETLTKFMQLHFPKTYLKD QHLSMLEYHVPVTAGGVANI FDLLETNKTALNITNFLVSQ TTLEEVFINFAKDQKSYETA DTSSQGSTISVDSQDDQMES |
| Tissue Specificity | Mainly expressed in the stomach, placenta, testis and fetal brain. Expressed in the upper epidermal layers, mainly the granular layers, of skin. Expressed throughout the normal interfollicular epidermis with prominent expression in the stratum granulosum. Expressed in alpha and beta cells of pancreatic islets. |
| Function | Transports lipids such as glucosylceramides from the outer to the inner leaflet of lamellar granules (LGs) membrane, whereby the lipids are finally transported to the keratinocyte periphery via the trans-Golgi network and LGs and released to the apical surface of the granular keratinocytes to form lipid lamellae in the stratum corneum of the epidermis, which is essential for skin barrier function. In the meantime, participates in the transport of the lamellar granules-associated proteolytic enzymes, in turn regulates desquamation and keratinocyte differentiation. Furthermore, is essential for the regulation of cellular cholesterol homeostasis by regulating ABCA1-dependent cholesterol efflux from macrophages through interaction with NR1H2 and ABCA1. Plays pleiotropic roles in regulating glucose stimulated insulin secretion from beta cells, regulating the morphology and fusion of insulin granules, lipid raft abundance and the actin cytoskeleton. Also involved in lung surfactant biogenesis. |
| Protein Name | Glucosylceramide Transporter Abca12Atp-Binding Cassette Sub-Family A Member 12Atp-Binding Cassette Transporter 12Atp-Binding Cassette 12 |
| Database Links | Reactome: R-HSA-1369062Reactome: R-HSA-5682294 |
| Cellular Localisation | Cytoplasmic VesicleSecretory Vesicle MembraneMulti-Pass Membrane ProteinGolgi Apparatus MembraneLocalizes In The Limiting Membrane Of The Lamellar Granules (Lgs)Trafficks From The Golgi Apparatus To The Lamellar Granules (Lgs) At The Cell Periphery In The Uppermost Granular Layer Keratinocytes Where Abca12-Positive Lgs Fuse With The Keratinocyte-Cell Membrane To Secrete Their Lipid Content To The Extracellular Space Of The Stratum CorneumCo-Localizes Through The Golgi Apparatus To The Cell Periphery With Glucosylceramide |
| Alternative Antibody Names | Anti-Glucosylceramide Transporter Abca12 antibodyAnti-Atp-Binding Cassette Sub-Family A Member 12 antibodyAnti-Atp-Binding Cassette Transporter 12 antibodyAnti-Atp-Binding Cassette 12 antibodyAnti-ABCA12 antibodyAnti-ABC12 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
