• Western blot analysis of extracts of various cell lines, using AAMP antibody (STJ26228) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of 293T cells using AAMP antibody (STJ26228) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AAMP antibody (85-434) (STJ26228)

SKU:
STJ26228

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AAMP (85-434) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:3000
IF/ICC 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AAMP
Gene ID: 14
Uniprot ID: AAMP_HUMAN
Immunogen Region: 85-434
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 85-434 of human AAMP (NP_001078.2).
Immunogen Sequence: VTFALHSASVFCVSLDPKTN TLAVTGGEDDKAFVWRLSDG ELLFECAGHKDSVTCAGFSH DSTLVATGDMSGLLKVWQVD TKEEVWSFEAGDLEWMEWHP RAPVLLAGTADGNTWMWKVP NGDCKTFQGPNCPATCGRVL PDGKRAVVGYEDGTIRIWDL KQGSPIHVLKGTEGHQGPLT CVAANQDGSLILTGSVDCQA KLVSATTGKVVGVFRPETVA SQPSLGEGEESESNSVESL
Tissue Specificity Expressed in metastatic melanoma, liver, skin, kidney, heart, lung, lymph node, skeletal muscle and brain, and also in A2058 melanoma cells and activated T-cells (at protein level). Expressed in blood vessels. Strongly expressed in endothelial cells, cytotrophoblasts, and poorly differentiated. colon adenocarcinoma cells found in lymphatics.
Function Plays a role in angiogenesis and cell migration. In smooth muscle cell migration, may act through the RhoA pathway.
Protein Name Angio-Associated Migratory Cell Protein
Database Links Reactome: R-HSA-168638
Reactome: R-HSA-177929
Reactome: R-HSA-194138
Reactome: R-HSA-428930
Cellular Localisation Cell Membrane
Cytoplasm
Alternative Antibody Names Anti-Angio-Associated Migratory Cell Protein antibody
Anti-AAMP antibody

Information sourced from Uniprot.org

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