• Western blot analysis of extracts of various cell lines, using AAMP antibody (STJ26228) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of 293T cells using AAMP antibody (STJ26228) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AAMP antibody (85-434) (STJ26228)


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Host: Rabbit
Applications: WB, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal antibody anti-AAMP (85-434) is suitable for use in Western Blot and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:3000
IF 1:100-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: AAMP
Gene ID: 14
Immunogen Region: 85-434
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 85-434 of human AAMP (NP_001078.2).
Tissue Specificity Expressed in metastatic melanoma, liver, skin, kidney, heart, lung, lymph node, skeletal muscle and brain, and also in A2058 melanoma cells and activated T-cells (at protein level). Expressed in blood vessels. Strongly expressed in endothelial cells, cytotrophoblasts, and poorly differentiated. colon adenocarcinoma cells found in lymphatics.
Function Plays a role in angiogenesis and cell migration. In smooth muscle cell migration, may act through the RhoA pathway.
Protein Name Angio-Associated Migratory Cell Protein
Database Links Reactome: R-HSA-168638
Reactome: R-HSA-177929
Reactome: R-HSA-194138
Reactome: R-HSA-428930
Cellular Localisation Cell Membrane
Alternative Antibody Names Anti-Angio-Associated Migratory Cell Protein antibody
Anti-AAMP antibody

Information sourced from Uniprot.org

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