• Immunohistochemistry analysis of AADAT in paraffin-embedded human liver using AADAT Rabbit polyclonal antibody (STJ115057) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using AADAT Rabbit polyclonal antibody (STJ115057) at 1:4000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AADAT antibody (34-429) (STJ115057)

SKU:
STJ115057

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AADAT (34-429) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:4000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AADAT
Gene ID: 51166
Uniprot ID: AADAT_HUMAN
Immunogen Region: 34-429
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 34-429 of human AADAT (NP_001273611.1).
Immunogen Sequence: PKSMISLAGGLPNPNMFPFK TAVITVENGKTIQFGEEMMK RALQYSPSAGIPELLSWLKQ LQIKLHNPPTIHYPPSQGQM DLCVTSGSQQGLCKVFEMII NPGDNVLLDEPAYSGTLQSL HPLGCNIINVASDESGIVPD SLRDILSRWKPEDAKNPQKN TPKFLYTVPNGNNPTGNSLT SERKKEIYELARKYDFLIIE DDPYYFLQFNKFRVPTFLSM DVDGRVIRADSFSKIISSG
Tissue Specificity Higher expression in the liver. Also found in heart, brain, kidney, pancreas, prostate, testis and ovary.
Function Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).
Protein Name Kynurenine/Alpha-Aminoadipate Aminotransferase - Mitochondrial
Kat/Aadat
2-Aminoadipate Aminotransferase
2-Aminoadipate Transaminase
Alpha-Aminoadipate Aminotransferase
Aadat
Glycine Transaminase Aadat
Kynurenine Aminotransferase Ii
Kynurenine--Glyoxylate Transaminase Aadat
Kynurenine--Oxoglutarate Aminotransferase Ii
Kynurenine--Oxoglutarate Transaminase 2
Kynurenine--Oxoglutarate Transaminase Ii
Methionine--Glyoxylate Transaminase Aadat
Database Links Reactome: R-HSA-71064
Reactome: R-HSA-71240
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Kynurenine/Alpha-Aminoadipate Aminotransferase - Mitochondrial antibody
Anti-Kat/Aadat antibody
Anti-2-Aminoadipate Aminotransferase antibody
Anti-2-Aminoadipate Transaminase antibody
Anti-Alpha-Aminoadipate Aminotransferase antibody
Anti-Aadat antibody
Anti-Glycine Transaminase Aadat antibody
Anti-Kynurenine Aminotransferase Ii antibody
Anti-Kynurenine--Glyoxylate Transaminase Aadat antibody
Anti-Kynurenine--Oxoglutarate Aminotransferase Ii antibody
Anti-Kynurenine--Oxoglutarate Transaminase 2 antibody
Anti-Kynurenine--Oxoglutarate Transaminase Ii antibody
Anti-Methionine--Glyoxylate Transaminase Aadat antibody
Anti-AADAT antibody
Anti-KAT2 antibody
Anti-KYAT2 antibody

Information sourced from Uniprot.org

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