• Western blot analysis of extracts of HepG2 cells, using Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M) ) rabbit monoclonal antibody (STJ11102540) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded rat lung using Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M) ) rabbit monoclonal antibody (STJ11102540) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human placenta using Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M) ) rabbit monoclonal antibody (STJ11102540) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M) ) rabbit monoclonal antibody (STJ11102540) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat liver using Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M) ) rabbit monoclonal antibody (STJ11102540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of human liver using Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M) ) rabbit monoclonal antibody (STJ11102540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse liver using Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M) ) rabbit monoclonal antibody (STJ11102540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-A2M antibody [ARC1734] (STJ11102540)

SKU:
STJ11102540

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit monoclonal antibody anti-A2M is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Clonality: Monoclonal
Clone ID: ARC1734
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: A2M
Gene ID: 2
Uniprot ID: A2MG_HUMAN
Immunogen: A synthesized peptide derived from human Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M) )
Tissue Specificity Secreted in plasma.
Function Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Protein Name Alpha-2-Macroglobulin
Alpha-2-M
C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 5
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-140837
Reactome: R-HSA-1474228
Reactome: R-HSA-8963896
Cellular Localisation Secreted
Alternative Antibody Names Anti-Alpha-2-Macroglobulin antibody
Anti-Alpha-2-M antibody
Anti-C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 5 antibody
Anti-A2M antibody
Anti-CPAMD5 antibody
Anti-FWP007 antibody

Information sourced from Uniprot.org

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