| Short Description : | Goat polyclonal antibody anti-38991 (192-204) is suitable for use in ELISA and Western Blot research applications. |
| Applications: | Pep-ELISA/WB |
| Host: | Goat |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 0.5 mg/mL |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 48kDa band observed in lysates of cell line A549 (calculated MW of 45.5kDa according to NP_002690.3). Recommended concentration: 1-3µg/ml. |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | POU3F1 |
| Gene ID: | 5453 |
| Uniprot ID: | PO3F1_HUMAN |
| Accession Number: | NP_002690.3 |
| Immunogen Region: | 192-204 |
| Immunogen Sequence: | HEDGHEAQLEPSP |
| Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters. Acts as a transcriptional repressor of myelin-specific genes. |
| Protein Name | Pou Domain - Class 3 - Transcription Factor 1Octamer-Binding Protein 6Oct-6Octamer-Binding Transcription Factor 6Otf-6Pou Domain Transcription Factor Scip |
| Database Links | Reactome: R-HSA-9619665Reactome: R-HSA-9823739Reactome: R-HSA-9832991 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Pou Domain - Class 3 - Transcription Factor 1 antibodyAnti-Octamer-Binding Protein 6 antibodyAnti-Oct-6 antibodyAnti-Octamer-Binding Transcription Factor 6 antibodyAnti-Otf-6 antibodyAnti-Pou Domain Transcription Factor Scip antibodyAnti-POU3F1 antibodyAnti-OCT6 antibodyAnti-OTF6 antibody |
Information sourced from Uniprot.org
![Immunofluorescence analysis of L929 cells using [KO Validated] NDUFS2 Rabbit polyclonal antibody (STJ114724) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/93811/375003/STJ114724_1__02265.1713141659.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] NDUFS2 Rabbit polyclonal antibody (STJ114724) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunoprecipitation analysis of 300 Mu g extracts of Hela cells using 3 Mu g [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11101180). Western blot was performed from the immunoprecipitate using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11101180) at a dilition of 1:1000.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/90102/358868/STJ11101180_1__04910.1713123234.jpg?c=1 "Immunoprecipitation analysis of 300 Mu g extracts of Hela cells using 3 Mu g [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11101180). Western blot was performed from the immunoprecipitate using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11101180) at a dilition of 1:1000.")
