| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Annexin A1 Blocking Peptide is synthetically produced from the 1-50 sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
ANXA1 |
| Gene ID: |
301 |
| Uniprot ID: |
ANXA1_HUMAN |
| Immunogen Region: |
1-50 |
| Immunogen: |
Synthetic peptide taken within amino acid region 1-50 on human annexin A1 protein. |
| Tissue Specificity | Detected in resting neutrophils. Detected in peripheral blood T-cells. Detected in extracellular vesicles in blood serum from patients with inflammatory bowel disease, but not in serum from healthy donors. Detected in placenta (at protein level). Detected in liver. |
| Post Translational Modifications | Phosphorylated by protein kinase C, EGFR and TRPM7. Phosphorylated in response to EGF treatment. Sumoylated. Proteolytically cleaved by cathepsin CTSG to release the active N-terminal peptide Ac2-26. |
| Function | Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Has no effect on unstimulated T cells. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton. Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions. Displays Ca(2+)-dependent binding to phospholipid membranes. Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton. Annexin Ac2-26: Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Promotes resolution of inflammation and wound healing. Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2. |
| Peptide Name | Annexin A1Annexin IAnnexin-1Calpactin IiCalpactin-2Chromobindin-9Lipocortin IPhospholipase A2 Inhibitory ProteinP35 Cleaved Into - Annexin Ac2-26 |
| Database Links | Reactome: R-HSA-416476Reactome: R-HSA-418594Reactome: R-HSA-444473Reactome: R-HSA-445355Reactome: R-HSA-6785807 |
| Cellular Localisation | NucleusCytoplasmCell ProjectionCiliumCell MembraneMembranePeripheral Membrane ProteinEndosome MembraneBasolateral Cell MembraneApical Cell MembraneLateral Cell MembraneSecretedExtracellular SpaceExtracellular SideExtracellular ExosomeCytoplasmic VesicleSecretory Vesicle LumenPhagocytic CupEarly EndosomeCytoplasmic Vesicle MembraneAt Least In Part Via Exosomes And Other Secretory VesiclesDetected In Exosomes And Other Extracellular VesiclesAlternativelyThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10It Results In The Protein Translocation From The Cytoplasm Into Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And SecretionDetected In Gelatinase Granules In Resting NeutrophilsSecretion Is Increased In Response To Wounding And InflammationSecretion Is Increased Upon T-Cell ActivationNeutrophil Adhesion To Endothelial Cells Stimulates Secretion Via Gelatinase GranulesBut Foreign Particle Phagocytosis Has No EffectColocalizes With Actin Fibers At Phagocytic CupsDisplays Calcium-Dependent Binding To Phospholipid Membranes |
| Alternative Peptide Names | Annexin A1 proteinAnnexin I proteinAnnexin-1 proteinCalpactin Ii proteinCalpactin-2 proteinChromobindin-9 proteinLipocortin I proteinPhospholipase A2 Inhibitory Protein proteinP35 Cleaved Into - Annexin Ac2-26 proteinANXA1 proteinANX1 proteinLPC1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
