| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
SPT5 Positive Control for STJ506404 is synthetically produced from the 760-810 sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Supt5h |
| Gene ID: |
20924 |
| Uniprot ID: |
SPT5H_MOUSE |
| Immunogen Region: |
760-810 |
| Specificity: |
This is positive control is recommended for use in combination with SPT5 antibody STJ506404. |
| Post Translational Modifications | Methylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II. Phosphorylated by CDK7 and CDK9. Phosphorylation by P-TEFb alleviates transcriptional pausing. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis. |
| Function | Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. |
| Peptide Name | Transcription Elongation Factor Spt5Drb Sensitivity-Inducing Factor Large SubunitDsif Large Subunit |
| Database Links | Reactome: R-MMU-112382Reactome: -MMU-113418Reactome: -MMU-674695Reactome: -MMU-6796648Reactome: -MMU-6807505Reactome: -MMU-72086Reactome: -MMU-75955Reactome: -MMU-77075 |
| Cellular Localisation | Nucleus |
| Alternative Peptide Names | Transcription Elongation Factor Spt5 proteinDrb Sensitivity-Inducing Factor Large Subunit proteinDsif Large Subunit proteinSupt5h proteinSupt5 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
