SPHK1 Blocking Peptide for STJ503118 peptide (STJ504957)

SKU:
STJ504957-250

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Applications: Immunodepletion/Immunocompetition
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: SPHK1 Blocking Peptide for STJ503118 is synthetically produced from the 50-100 sequence and is suitable for use in western blot applications.
Formulation: Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: Sphk1
Gene ID: 20698
Uniprot ID: SPHK1_MOUSE
Immunogen Region: 50-100
Specificity: This blocking peptide is recommended for use in combination with SPHK1 antibody, STJ503118
Immunogen: Synthetic peptide taken within amino acid region 50-100 on human SPHK1 protein.
Tissue Specificity Widely expressed. Expressed in brain (at protein level). Detected in neurons.
Function Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis. Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling. In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway. Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity. In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA. Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia.
Peptide Name Sphingosine Kinase 1
Sk 1
Spk 1
Acetyltransferase Sphk1
Database Links Reactome: R-MMU-1660661
Reactome: -MMU-390471
Reactome: -MMU-5218921
Reactome: -MMU-9009391
Cellular Localisation Cytoplasm
Endosome Membrane
Peripheral Membrane Protein
Nucleus
Cell Membrane
Synapse
Translocated From The Cytoplasm To The Plasma Membrane In A Cib1-Dependent Manner
Binds To Membranes Containing Negatively Charged Lipids But Not Neutral Lipids
Recruited To Endocytic Membranes By Sphingosine Where Promotes Membrane Fusion
Alternative Peptide Names Sphingosine Kinase 1 protein
Sk 1 protein
Spk 1 protein
Acetyltransferase Sphk1 protein
Sphk1 protein
Sk1 protein

Information sourced from Uniprot.org

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