| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
SHANK3 Positive Control for STJ502998 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Shank3 |
| Gene ID: |
58234 |
| Uniprot ID: |
SHAN3_MOUSE |
| Specificity: |
This is positive control is recommended for use in combination with SHANK3 antibody STJ502998. |
| Tissue Specificity | In brain, highly expressed in striatum, thalamus, hippocampus and granule cells of the cerebellum. |
| Function | Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation. |
| Peptide Name | Sh3 And Multiple Ankyrin Repeat Domains Protein 3Shank3Proline-Rich Synapse-Associated Protein 2Prosap2Spank-2 |
| Database Links | Reactome: R-MMU-6794361Reactome: -MMU-8853659 |
| Cellular Localisation | CytoplasmSynapsePostsynaptic DensityCell ProjectionDendritic SpineIn Neuronal CellsExtends Into The Region Subjacent To The Postsynaptic Density (Psd) |
| Alternative Peptide Names | Sh3 And Multiple Ankyrin Repeat Domains Protein 3 proteinShank3 proteinProline-Rich Synapse-Associated Protein 2 proteinProsap2 proteinSpank-2 proteinShank3 proteinKiaa1650 proteinProsap2 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
