Applications: | WB |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | SFN Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
Formulation: | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
Dilution Range: | WB: 1:2, 500-1:5, 000 |
Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Gene Symbol: | SFN |
Gene ID: | 2810 |
Uniprot ID: | 1433S_HUMAN |
Tissue Specificity | Present mainly in tissues enriched in stratified squamous keratinizing epithelium. |
Post Translational Modifications | Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation. |
Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response. Also acts as a TP53/p53-regulated inhibitor of G2/M progression. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis. Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. |
Peptide Name | 14-3-3 Protein Sigma Epithelial Cell Marker Protein 1 Stratifin |
Database Links | Reactome: R-HSA-111447 Reactome: R-HSA-1445148 Reactome: R-HSA-5625740 Reactome: R-HSA-5628897 Reactome: R-HSA-6804114 Reactome: R-HSA-75035 Reactome: R-HSA-9614399 Reactome: R-HSA-9735871 Reactome: R-HSA-9755779 |
Cellular Localisation | Cytoplasm Nucleus Secreted May Be Secreted By A Non-Classical Secretory Pathway |
Alternative Peptide Names | 14-3-3 Protein Sigma protein Epithelial Cell Marker Protein 1 protein Stratifin protein SFN protein HME1 protein |
Information sourced from Uniprot.org