| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
SFN Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:2, 500-1:5, 000 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Tissue Specificity | Present mainly in tissues enriched in stratified squamous keratinizing epithelium. |
| Post Translational Modifications | Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation. |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. p53-regulated inhibitor of G2/M progression. |
| Peptide Name | 14-3-3 Protein SigmaEpithelial Cell Marker Protein 1Stratifin |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-1445148Reactome: R-HSA-5625740Reactome: R-HSA-5628897Reactome: R-HSA-6804114Reactome: R-HSA-75035Reactome: R-HSA-9614399Reactome: R-HSA-9735871Reactome: R-HSA-9755779 |
| Cellular Localisation | CytoplasmNucleusSecretedMay Be Secreted By A Non-Classical Secretory Pathway |
| Alternative Peptide Names | 14-3-3 Protein Sigma proteinEpithelial Cell Marker Protein 1 proteinStratifin proteinSFN proteinHME1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
