| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-SARS COV2 Spike protein S-Fc-Tag protein was developed from hek293 and has a target region of Fc-Tag. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.2 µm filtered PBS pH 7.4. |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunogen Region: | ECD |
| Immunogen: | DNA sequence encoding the COVID-19 Spike S protein extracellular domain, [14-1211] accession #QHD43416.1 including a C-terminal Fc AVI tag was expressed in HEK Cells. (The furin protease cleavage site was mutated at position 683) NA |
| Background | Recombinant 2019-nCoV Spike S protein is a protein consisting of the S1+S2 subunits with a calculated MW of 160kDa, due to glycosylation migrates as an approximately 220 kDa protein on SDS-PAGE. |
Information sourced from Uniprot.org