| Short Description : | Active Recombinant-SARS-CoV-2 Spike S1+S2 ECD (S-ECD) (D614G)-C-His protein was developed in hek293 cells using the region Val11-Gln1208Asp614Gly. For use in research applications. |
| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Supplied as a 0.22 Mu m filtered solution in PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike S1+S2 ECD at 2 Mu g/mL (100 Mu L/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.55 ng/mL. |
| Determination Method: | < 1.0 EU/Mu g of the protein by LAL method. |
| Immunogen: | Recombinant SARS-CoV-2 Spike S1+S2 ECD (S-ECD) (D614G) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Gln1208 (Asp614Gly) ) of sars-cov-2 S1+S2 ECD ( S-ECD ) (D614G) (Accession #YP_00972439 |
| Immunogen Region: | Val11-Gln1208Asp614Gly |
| Immunogen Sequence: | VSSQCVNLTTRTQLPPAYTN SFTRGVYYPDKVFRSSVLHS TQDLFLPFFSNVTWFHAIHV SGTNGTKRFDNPVLPFNDGV YFASTEKSNIIRGWIFGTTL DSKTQSLLIVNNATNVVIKV CEFQFCNDPFLGVYYHKNNK SWMESEFRVYSSANNCTFEY VSQPFLMDLEGKQGNFKNLR EFVFKNIDGYFKIYSKHTPI NLVRDLPQGFSALEPLVDLP IGINITRFQTLLALHRSYL |
| Background | Active Recombinant SARS-CoV-2 Spike S1+S2 ECD (S-ECD) (D614G) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Gln1208 (Asp614Gly) ) of sars-cov-2 S1+S2 ECD ( S-ECD ) (D614G) (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus. |
Information sourced from Uniprot.org
