Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of extracts of RAW264. 7 cells, using IL2RB antibody (STJ27963) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IL2RB antibody (316-431) (STJ27963)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of HeLa cells, using IL-2R beta Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-IL2RB antibody (331-380 aa) (STJ93696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of K562 cells using IL-2R Gamma Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL2RG antibody (101-150 aa) (STJ96745)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using CD132/IL-2 R gamma antibody (STJ11104677) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-IL2RG antibody (250-350) [S4677RM] (STJ11104677)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using CD132/IL-2 R gamma antibody (STJ24184) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-IL2RG antibody (290-369) (STJ24184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using IL3 antibody (STJ27638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-IL3 antibody (20-152) (STJ27638)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-IL3 antibody (STJA0010239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-IL3 antibody [SAA1520] (STJA0019948)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Western blot analysis of various cells using IL-32 Polyclonal Antibody

Anti-IL32 antibody (177-226 aa) (STJ93697)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot with Anti-IL32 Antibody (STJ501446) with peptide on at 1:500 dilution in buffer.

Anti-IL32 antibody (180-234) (STJ501446)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-IL32 antibody (180-234) {Biotin} (STJ501447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-IL32 antibody (180-234) {FITC} (STJ501448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-IL32 antibody [SAA1547] (STJA0019975)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Anti-IL33 antibody (109-270 aa) [SRM5370] (STJ11107280)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£126.50 - £554.50

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Western blot analysis of various lysates using IL33 Rabbit pAb (STJ110395) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-IL33 antibody (110-190) (STJ110395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of HeLa cells using IL-33 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL33 antibody (121-170 aa) (STJ96852)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-IL33 antibody [C2_2E12] (STJA0018524)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Blocking/ELISA/WB

Reactivity :

Human

£198.50 - £1,170.50

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Anti-IL33 antibody [SAA0395] (STJA0027574)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£286.50

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Anti-IL33 antibody [SAA0395] {APC} (STJA0028929)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-IL33 antibody [SAA0395] {FITC} (STJA0027963)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£284.50

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Anti-IL33 antibody [SAA0395] {PerCP} (STJA0029895)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£330.50

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Anti-IL33 antibody [SAA0395] {PE} (STJA0030861)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-IL33 antibody [SRM14549] (STJ11109279)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using IL3RA antibody (STJ24185) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-IL3RA antibody (1-100) (STJ24185)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of Mouse spleen, using CD123 Rabbit polyclonal antibody (STJ11104716) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL3RA antibody (20-305) (STJ11104716)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£110.50 - £505.50

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Western blot analysis of HepG2 cells using IL-3R Alpha Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL3RA antibody (241-290 aa) (STJ97265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using IL5 Rabbit pAb (STJ110003) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 15s.

Anti-IL5 antibody (20-134) (STJ110003)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of recombinant Human IL-5 Protein, using Human IL-5 Rabbit polyclonal antibody (STJ11104218) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 8s.

Anti-IL5 antibody (20-134) (STJ11104218)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£110.50 - £505.50

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Western blot analysis of various cells using IL-5 Polyclonal Antibody

Anti-IL5 antibody (43-92 aa) (STJ93704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-IL5 antibody [SAA0372] (STJA0027551)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£98.50

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Anti-IL5 antibody [SAA0372] {APC} (STJA0029012)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£201.50

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Anti-IL5 antibody [SAA0372] {FITC} (STJA0028046)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£187.50

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Anti-IL5 antibody [SAA0372] {PerCP} (STJA0029978)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£194.50

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Anti-IL5 antibody [SAA0372] {PE} (STJA0030944)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£194.50

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Anti-IL5 antibody [SAA0373] (STJA0027552)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£286.50

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Anti-IL5 antibody [SAA0373] {APC} (STJA0029013)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-IL5 antibody [SAA0373] {FITC} (STJA0028047)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£284.50

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Anti-IL5 antibody [SAA0373] {PerCP} (STJA0029979)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£330.50

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Anti-IL5 antibody [SAA0373] {PE} (STJA0030945)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-IL5 antibody [SAA0374] (STJA0027553)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£286.50

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Anti-IL5 antibody [SAA0374] {APC} (STJA0029014)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-IL5 antibody [SAA0374] {FITC} (STJA0028048)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£284.50

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Anti-IL5 antibody [SAA0374] {PerCP} (STJA0029980)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£330.50

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Anti-IL5 antibody [SAA0374] {PE} (STJA0030946)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Western blot analysis of various lysates using IL5RA Rabbit pAb (STJ111288) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-IL5RA antibody (20-335) (STJ111288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of K562 cells using IL-5R Alpha Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL5RA antibody (41-90 aa) (STJ96579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from HUVEC cells, using IL6 Rabbit monoclonal antibody (STJ11104756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-IL6 antibody (1-212) [S4756RM] (STJ11104756)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, IL-6 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IL6 antibody (131-180 aa) (STJ96713)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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