Protein Localisation | Research Area | St John's Laboratory

Anti-HSPA9/Mortalin/GRP75 antibody [SAA0812] (STJA0019624)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 1 Mu g HSP60/HSPD1 antibody (STJ24105). Western blot was performed from the immunoprecipitate using HSP60/HSPD1 antibody (STJ24105) at a dilution of 1:1000.

Anti-HSPD1 antibody (27-240) (STJ24105)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of HeLa cells using HSP60/HSPD1 Rabbit monoclonal antibody (STJ11101241, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Rabbit monoclonal antibody ( dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-HSPD1 antibody (350-450) [S1MR] (STJ11101241)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Wheat

£146.50 - £642.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-HSP60 antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 60 kDa

Anti-HSPD1 antibody (500-573 aa) [ABT186] (STJ197164)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, HSP60 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-HSPD1 antibody (511-560 aa) (STJ93621)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~60 kDa HSP60 protein.

Anti-HSPD1 antibody (Full Length) [1C7] (STJA0003845)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep/Zebrafish

£454.50

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Anti-HSPD1 antibody (N-Term) [M438] (STJA0005440)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£390.50

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Western blot detection of HSP60 in pc-12, COS7, 3T3, Hela Rat kidney, Rat Skeletal muscle Rat lung and T47D cell lysates using HSP60 antibody (1:500 diluted).Predicted band size:60KDa.Observed band size:60KDa.

Anti-HSPD1 antibody (STJ99294)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Western blot analysis of 1) 293 Cell, 2) Mouse Kidney Tissue, 3) Rat Heart Tissue lysate using HSP60 mouse monoclonal antibody diluted at 1:2,000.

Anti-HSPD1 antibody [2D3] (STJ196695)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis using HSP60 monoclonal antibody against T47D (1) , HeLa (2) , HepG2 (3) , A549 (4) , Jurkat (5) , HEK293 (6) , NIH/3T3 (7) , PC-12 (8) and Cos7 (9) cell lysate.

Anti-HSPD1 antibody [3G8] (STJ98142)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Western blot detection of Hsp60 in Hela, Raji, Jurkat, C6, C2C12 and 3T3 cell lysates using Hsp60 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.

Anti-HSPD1 antibody [6C8-G1-B10] (STJ99241)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Western blot analysis of extracts of various cell lines, using Insulin-degrading enzyme (Insulin-degrading enzyme (IDE) ) Rabbit monoclonal antibody (STJ11101287) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-IDE antibody (1-100) [S7MR] (STJ11101287)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of A549 cells using Insulin-degrading enzyme (Insulin-degrading enzyme (IDE) ) antibody (STJ24120). Blue: DAPI for nuclear staining.

Anti-IDE antibody (1-250) (STJ24120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Insulin Degrading Enzyme/IDE antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-IDE antibody (56-69 aa) [R07-6D3] (STJA0012660)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

£264.50

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Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, IDE monoclonal antibody (3H4) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IDE antibody [3H4] (STJ96985)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Hamster

£48.00 - £252.00

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Anti-IDE antibody [SAA1582] (STJA0020010)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g IDH1 antibody (STJ115211). Western blot was performed from the immunoprecipitate using IDH1 antibody (STJ115211) at a dilution of 1:1000.

Anti-IDH1 antibody (1-414) (STJ115211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of PC-12 cells using IDH1 Rabbit polyclonal antibody (STJ24121) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IDH1 antibody (100-414) (STJ24121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£112.50 - £524.50

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Western blot analysis of 293, HeLa cells using IDH1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IDH1 antibody (N-Term) (STJ96781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Formalin-fixed, paraffin-embedded human glioma stained with anti-IHD1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear and cytoplasmic staining of infiltrating glioma cells whereas normal glial cells are negative

Anti-IDH1 antibody [ZR7] (STJ180518)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£202.50 - £814.50

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Anti-IDH1-R132G-specific antibody [GMab-1] (STJA0022479)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-IDH1-R132H-specific antibody [HMab-1] (STJA0022481)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-IDH1-R132L-specific antibody [LMab-1] (STJA0022480)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-IDH1-R132S-specific antibody [SMab-1] (STJA0022482)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-IDH3G antibody (STJ193072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Confocal imaging of RAW264. 7 LPS and RAW264. 7 cells using iNOS Rabbit monoclonal antibody (STJ11103535, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-iNOS antibody (2-200) [S5MR] (STJ11103535)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£146.50 - £642.50

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Anti-iNOS antibody [SM0487] (STJA0010487)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Mouse

£170.50 - £233.50

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Anti-iNOS antibody [SM0501] (STJA0010501)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-K11-Linked Polyubiquitin-Specific antibody [2A3/2E6] (STJA0022260)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£378.50 - £1,632.50

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Anti-K48-linked Polyubiquitin antibody [SAA0329] (STJA0019256)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat/Rabbit

£378.50 - £1,632.50

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Anti-K63-linked Polyubiquitin antibody [SAA0330] (STJA0019257)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/IF/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of 1) Hela, 2) Mouse Skeletal Muscle, 3) Mouse Liver, 4) Rat Kidney, diluted at 1:5000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-LDHD antibody (STJ197354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using LONP2 Rabbit polyclonal antibody (STJ118354) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-LONP2 antibody (1-300) (STJ118354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using LONP2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-LONP2 antibody (780-829 aa) (STJ93944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Mortalin antibody (N-Term) (STJ13100184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse/Marmoset

£331.50

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Anti-Mortalin antibody (N-Term) (STJ13100188)

Clonality :

Polyclonal

Host :

Guinea Pig

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse/Marmoset

£331.50

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MPV17 antibody (45-95 aa) (STJ194570)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using MTX1 Rabbit pAb (STJ110221) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 90s.

Anti-MTX1 antibody (186-435) (STJ110221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-MTX1 antibody (STJ11107016)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-MTX1 antibody (STJA0008183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of various lysates using MTX2 Rabbit mAb (STJ11107347) at 1:3000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Negative control (NC) : REH<br>Exposure time: 30s.

Anti-MTX2 antibody (1-263 aa) [SRM] (STJ11107347)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates, using MTX2 Rabbit pAb (STJ110265) at 1:2000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-MTX2 antibody (1-263) (STJ110265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-MTX2 antibody (STJA0007707)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using NDUFB8 rabbit monoclonal antibody (STJ11103099) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NDUFB8 antibody (1-150) [S9MR] (STJ11103099)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NDUFB8 antibody (120-200 aa) (STJ191034)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using NDUFB8 Rabbit polyclonal antibody (STJ11103314) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NDUFB8 antibody (29-186) (STJ11103314)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot with Anti-NOS2 Antibody (STJ503603) on 1) RAW cells treated with LPS, 2) RAW cells. Antibody at 1:250 dilution in buffer.

Anti-NOS2 antibody (100-150) (STJ503603)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-NOS2 antibody (100-150) {Biotin} (STJ503605)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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