Programmed Cell Death | Research Area | St John's Laboratory

Western blot analysis of lysates from Mouse brain, using Phospho-Tau-S396 Rabbit mAb (STJ11102609) at 1:1000 dilution. Mouse brain were treated by CIP (20 Mu L/400 Mu L) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-MAPT-S396 antibody [S9MR] (STJ11102609)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of lysates from SH-SY5Y and C6 cells, using Phospho-Tau-S404 Rabbit pAb (STJ22272) at 1:2000 dilution. SH-SY5Y cells were treated by Okadaic Acid (100nM) for 1 hour and Calyculin A (100nM) for 30 minutes after serum-starvation overnight. C6 cell lysates were treated by CIP at 37°C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-MAPT-S404 antibody (STJ22272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from SH-SY5Y cells using Phospho-Tau-S404 Rabbit monoclonal antibody (STJ11105855) at 1:5000 dilution. SH-SY5Y cells were treated by Okadaic Acid (100 nM) at 37 °C for 1 hour and Calyculin A (100nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 30 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-Phospho-MAPT-S404 antibody [S5855RM] (STJ11105855)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Tau-Ser198/Ser199/Ser202 pAb (STJ11101130) at 1:1000 dilution or Tau antibody (STJ24499). NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 120s.

Anti-Phospho-MAPT-Ser198/Ser199/Ser202 antibody (STJ11101130)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Anti-Phospho-MAPT-Ser202 antibody (STJ196448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from HeLa cells, using Tau (Phospho-Ser214) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MAPT-Ser214 antibody (501-550 aa) (STJ90419)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-Tau (S235) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-MAPT-Ser235 antibody (526-575 aa) (STJ90420)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from HeLa cells, using Tau (Phospho-Ser262) Antibody. The lane on the left is blocked with the phospho peptide.

Anti-Phospho-MAPT-Ser262 antibody (551-600 aa) (STJ90421)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of NIH-3T3 cells using Phospho-Tau (S356) Polyclonal Antibody diluted at 1：500

Anti-Phospho-MAPT-Ser356 antibody (641-690 aa) (STJ90422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of Hela cell. 1, Tau (phospho Ser396) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-MAPT-Ser396 antibody (681-730 aa) (STJ90423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-MAPT-Ser396 antibody (STJ99598)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-Tau (S404) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-MAPT-Ser404 antibody (691-740 aa) (STJ90424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of paraffin-embedded Rat brain using Phospho-Tau-Ser404 Rabbit pAb (STJ11101134) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Phospho-MAPT-Ser404 antibody (STJ11101134)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Tau-Ser416 Rabbit pAb (STJ24499). NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MAPT-Ser416 antibody (STJ11101135)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Anti-Phospho-MAPT-Ser416 antibody (STJ196449)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human brain, using Tau (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MAPT-Ser422 antibody (706-755 aa) (STJ91005)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of Tau (Phospho-Ser516/199) Antibody. The lane on the right is blocked with the Tau (Phospho-Ser516/199) peptide.

Anti-Phospho-MAPT-Ser516/199 antibody (166-215 aa) (STJ90658)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from Jurkat cells treated with H2O2 100uM 30', using Tau (Phospho-Ser519/202) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MAPT-Ser519 antibody (266-315 aa) (STJ90576)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western Blot analysis of mouse brain, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-MAPT-Ser717/400 antibody (STJA0010037)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from 293T cells transfected with CDK5+CDK5R1+Tau and CDK5+CDK5R1+mutant Tau (T181/T217A) using Phospho-Tau-T181 Rabbit monoclonal antibody (STJ11105822) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-MAPT-T181 antibody [S5822RM] (STJ11105822)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of lysates from Rat brain, using Phospho-Tau-T205 Rabbit pAb (STJ22270) at 1:2000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-MAPT-T205 antibody (STJ22270)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from HeLa cells, using Phospho-Tau-T212 Rabbit pAb (STJ11105779) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-MAPT-T212 antibody (STJ11105779)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of various lysates using Phospho-Tau-T231 Rabbit pAb (STJ11105780) at 1:2000 dilution. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/> Lysates/ proteins: 25 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 60s.

Anti-Phospho-MAPT-T231 antibody (STJ11105780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$174.57 - $853.53

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Confocal imaging of paraffin-embedded Rat brain tissue using Phospho-Tau-T231 Rabbit mAb (STJ11102551, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-Phospho-MAPT-T231 antibody [S1MR] (STJ11102551)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

$163.53 - $719.67

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Western blot analysis of lysates from HeLa cells, using Phospho-Tau-T181 Rabbit pAb (STJ11101032) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-Phospho-MAPT-T498 antibody (STJ11101032)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of 293 cells using Phospho-Tau (T181) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-MAPT-Thr181 antibody (471-520 aa) (STJ90425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of VEC 293 lysis using Phospho-Tau (T205) antibody. Antibody was diluted at 1:2000

Anti-Phospho-MAPT-Thr205 antibody (491-540 aa) (STJ90426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human brain, using Tau (Phospho-Thr212) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MAPT-Thr212 antibody (496-545 aa) (STJ91285)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from Mouse brain, using Phospho-Tau-Thr212/Ser214 Rabbit pAb (STJ11101159) at 1:1000 dilution. Mouse brain were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-MAPT-Thr212/Ser214 antibody (STJ11101159)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Western blot analysis of lysates from Mouse brain, using Phospho-Tau-T231 Rabbit pAb (STJ11101057) at 1:1000 dilution. Mouse brain were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-MAPT-Thr231 antibody (STJ11101057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$174.57 - $853.53

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Western blot analysis of lysates from MDA-MB-435 cells, using Tau (Phospho-Thr231) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MAPT-Thr231 antibody (STJ90427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from rat brain, using Tau (Phospho-Thr534/217) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-MAPT-Thr534 antibody (181-230 aa) (STJ90577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-MLKL antibody (350-400) (STJ506097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-Phospho-MLKL antibody (350-400) {Biotin} (STJ506098)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$761.07

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Anti-Phospho-MLKL antibody (350-400) {FITC} (STJ506099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$761.07

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Anti-Phospho-MLKL-S358 antibody (STJ11108414)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-Phospho-MLKL-S358 antibody (STJ193224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, phospho-MLKL (S358) Mouse monoclonal antibody (6F8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Phospho-MLKL-S358 antibody [6F8] (STJ97776)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC

Reactivity :

Human

$67.62 - $360.18

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Anti-Phospho-MLKL-Ser345 antibody [PT0987R] (STJA0039926)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$393.30 - $745.20

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Anti-Phospho-MLKL-Ser358 antibody [R02-2I5] (STJA0036596)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

$184.23 - $388.47

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Anti-Phospho-OGT-Thr454 antibody (STJA0009873)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-p53-S15-S15 antibody [SRM15140] (STJ11109870)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$0.00

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Anti-Phospho-p53-Ser15 antibody [PT0901R] (STJA0039935)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Anti-Phospho-p53-Ser33 antibody [R04-1B1] (STJA0035772)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

$184.23 - $388.47

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Anti-Phospho-p53-Ser376 antibody [R06-1Y5] (STJA0035935)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$184.23 - $388.47

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Anti-Phospho-p53-Ser392 antibody (STJA0037463)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-Phospho-p53-Ser392 antibody [PT1223R] (STJA0040171)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Anti-Phospho-p53-Ser392 antibody [R08-2A8] (STJA0035832)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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