Immune System | Research Area | St John's Laboratory

Western blot analysis of mouse-lung mouse-heart mouse-liver lysis using EDA antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-EDA antibody (120-170 aa) (STJ97656)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of HepG2 cells, using EDA antibody (STJ23467) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-EDA antibody (62-182) (STJ23467)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-EDA1 antibody [Fs0331] (STJA0032135)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FCM/WB

Reactivity :

Human

£367.50

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-EDA2R antibody (STJ192277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using EDAR antibody (STJ23468) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EDAR antibody (27-187) (STJ23468)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-EDAR antibody (50-130 aa) (STJ190407)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-EDAR/DL antibody [SAA1633] (STJA0020061)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Western blot analysis of various lysates using EDARADD Rabbit polyclonal antibody (STJ118395) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-EDARADD antibody (1-215) (STJ118395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of lysates from mouse pancreas, using EDARADD Rabbit polyclonal antibody (STJ118396) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EDARADD antibody (1-215) (STJ118396)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Anti-EDARADD antibody (108-158) (STJA0006542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-EDB/EDB-FN antibody [SAA1375] (STJA0019667)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Blocking/ELISA/IHC/WB

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of extracts of various cell lines, using EEA1 Rabbit monoclonal antibody (STJ11102233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EEA1 antibody (1-100) [S3MR] (STJ11102233)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded Mouse kidney using EEA1 antibody (STJ110997) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-EEA1 antibody (1182-1411) (STJ110997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Endogenous EEA1 detected with antibody at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-EEA1 antibody (1230aa C-Term) (STJ140033)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Anti-EEA1 antibody at 1:500 dilution; Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-EEA1 antibody (1230aa C-Term) (STJ140034)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Western blot analysis of extracts from K562 cells, using EEA1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-EEA1 antibody (205-254 aa) (STJ96393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-EEA1 antibody (N-Term) [M347] (STJA0005387)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£390.50

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Anti-EEA1 antibody [PT0188R] (STJA0039703)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunofluorescence analysis of U-2 OS cells using EED Rabbit polyclonal antibody (STJ114646) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EED antibody (1-100) (STJ114646)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using EED rabbit polyclonal antibody (STJ27324) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-EED antibody (1-400) (STJ27324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-EED antibody (STJA0006915)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of U2OS cells using eEF1A1 Rabbit polyclonal antibody (STJ23475) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EEF1A1 antibody (123-462) (STJ23475)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Confocal imaging of C6 cells using eEF1A1 Rabbit monoclonal antibody (STJ11101355, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-EEF1A1 antibody (250-350) [S5MR] (STJ11101355)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of extracts of various cell lines, using eEF1A1 antibody (STJ119870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-EEF1A1 antibody (301-400) (STJ119870)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunocytochemistry analysis of Elongation Factor 1A1 (green) in Hela using Elongation Factor 1A1 antibody, and DAPI (blue).

Anti-EEF1A1 antibody (325-338 aa) [R04-3H4] (STJA0012354)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Rat

£264.50

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Immunohistochemistry analysis of paraffin-embedded Human tonsil using eEF1A1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-EEF1A1 antibody (434-447 aa) [R05-4K5] (STJA0011256)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Anti-eEF1A1 antibody [SRM14654] (STJ11109384)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of extracts of 293T cells, using eEF2 antibody (STJ114864) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-EEF2 antibody (1-290) (STJ114864)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using eEF2 antibody (STJ23480) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-EEF2 antibody (1-290) (STJ23480)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of various cells using EF-2 Polyclonal Antibody diluted at 1：2000

Anti-EEF2 antibody (31-80 aa) (STJ92841)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using eEF2 Rabbit mAb (STJ11102532) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-EEF2 antibody (759-858) [S2MR] (STJ11102532)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis using EF-2 monoclonal antibody against HL-60 cell lysate.

Anti-EEF2 antibody (STJ98488)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£149.00 - £360.00

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Western blot detection of eEF2 in HL-60, Jurkat, SHSY-5Y, U20S and Hela cell lysates using eEF2 mouse mAb (1:5000 diluted).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s.

Anti-EEF2 antibody [4B3-G7-H5] (STJ99117)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-eEF2 antibody [PT0594R] (STJA0040234)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunofluorescence analysis of U2OS cells using EGR1 Rabbit polyclonal antibody (STJ23496) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-EGR1 antibody (1-250) (STJ23496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from ECV-304 cells, using EGR1 Rabbit pAb (STJ29407) at 1:1000 dilution. ECV-304 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-EGR1 antibody (1-250) (STJ29407)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £618.50

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Western blot analysis using Egr-1 monoclonal antibody against EGR1-hIgGFc transfected HEK293 (1) cell lysate.

Anti-EGR1 antibody (282-433 aa) [8A6] (STJ98014)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-EGR1 antibody [PT0584R] (STJA0039249)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Western blot analysis of various lysates, using PKR/EIF2AK2 Rabbit polyclonal antibody (STJ27547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 3s.

Anti-EIF2AK2 antibody (1-270) (STJ27547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of various cells using PKR Polyclonal Antibody diluted at 1：2000

Anti-EIF2AK2 antibody (413-462 aa) (STJ95144)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using PKR/EIF2AK2 antibody (STJ11101690) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EIF2AK2 antibody (450-551) [S0MR] (STJ11101690)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunofluorescence analysis of MCF7 cells using PERK Rabbit polyclonal antibody (STJ11105581) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EIF2AK3 antibody (50-600) (STJ11105581)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunofluorescence analysis of NIH/3T3 cells using PERK Rabbit polyclonal antibody (STJ11100155) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EIF2AK3 antibody (867-1116) (STJ11100155)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of Hela cell. 1, PERK Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-EIF2AK3 antibody (947-996 aa) (STJ95031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of CACO2 lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-EIF2AK3 antibody (STJ99624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using PERK monoclonal antibody against HEK293 (1) and EIF2AK3-hIgGFc transfected HEK293 (2) cell lysate.

Anti-EIF2AK3 antibody [5G5] (STJ98319)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£149.00 - £360.00

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Immunohistochemistry analysis of paraffin-embedded human lung cancer using eIF2C3 Rabbit monoclonal antibody (STJ11103159) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-eIF2C3 antibody (721-860) [S9MR] (STJ11103159)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates, using eIF2 Alpha antibody (STJ23509) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-EIF2S1 antibody (1-260) (STJ23509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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