Gene Expression And Transcription | Research Area | St John's Laboratory

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g E2F6 rabbit monoclonal antibody (STJ11101356) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of E2F6 in representative gene loci (BRCA1) , as shown in figure.

Anti-E2F6 antibody (110-281) [S6MR] (STJ11101356)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA/ChIP/CUT&Tag

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of various cells using E2F-6 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-E2F6 antibody (141-190 aa) (STJ92811)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-E2F6 antibody [R09-2A4] (STJA0036816)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human

£133.50 - £281.50

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Anti-E2F7 antibody (560-640 aa) (STJ191781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using E2F8 Rabbit polyclonal antibody (STJ23459) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Anti-E2F8 antibody (788-867) (STJ23459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-E2F8 antibody (STJ191922)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using EAF1 Rabbit polyclonal antibody (STJ119827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-EAF1 antibody (50-120) (STJ119827)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Anti-EAF1 antibody (STJA0007298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-EAF2 antibody (STJA0009552)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of U-2 OS cells using EED Rabbit polyclonal antibody (STJ114646) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EED antibody (1-100) (STJ114646)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using EED rabbit polyclonal antibody (STJ27324) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-EED antibody (1-400) (STJ27324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-EED antibody (STJA0006915)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-EED IP antibody [SAA0245] (STJA0018760)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP/IP-MS

Reactivity :

Human

£195.50 - £1,319.50

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Western blot analysis of various lysates, using EGF Receptor (E746-A750del Specific) Rabbit pAb (STJ11106934) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-EGF Receptor-E746-A750del Specific antibody (STJ11106934)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of A431, using EGFR rabbit polyclonal antibody (STJ113093) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-EGFR antibody (1021-1210) (STJ113093)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of A-431 cells using [KD Validated] EGFR Rabbit polyclonal antibody (STJ113840) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EGFR antibody (1021-1210) (STJ113840)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Immunocytochemistry analysis of EGFR (green) in 3T3 using EGFR antibody, and DAPI (blue).

Anti-EGFR antibody (1075-1093 aa) [R01-2D3] (STJA0012677)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse

£140.50 - £308.50

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Immunocytochemistry analysis of EGFR (green) in Hela using EGFR antibody, and DAPI (blue)

Anti-EGFR antibody (1075-1093 aa) [R03-6B1] (STJA0013018)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse

£140.50 - £308.50

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Anti-EGFR antibody (1075-1093 aa) [R09-7I5] (STJA0011260)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£246.50

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Western blot analysis of extracts of Rat brain, using EGFR antibody (STJ113559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-EGFR antibody (1100-1200) (STJ113559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-ERBB1 antibody at 1:2500 dilution 50 µg of total protein per lane rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-EGFR antibody (1162aa C-Term) (STJ140129)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-P/IHC-F

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian

£135.50 - £440.50

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Human appendix tissue was stained with Anti-EGFR (PTR2121) Antibody

Anti-EGFR antibody (300-400) [PTR2121] (STJA0006243)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£49.00 - £261.00

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Western blot analysis of lysates from wild type (WT) and EGFR knockdown (KD) HeLa cells, using [KD Validated] EGFR Rabbit monoclonal antibody (STJ11105117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EGFR antibody (320-420 aa) [S5117RM] (STJ11105117)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma stained with anti-EGFR antibody using peroxidase-conjugate and DAB chromogen. Note the membrane staining of carcinoma cells

Anti-EGFR antibody (344-492aa) [ZR16] (STJ180567)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£155.50 - £604.50

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Anti-EGFR antibody (424-605aa) [AT2H8] (STJA0041614)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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Anti-EGFR antibody (424-605aa) [AT2H8] (STJA0041914)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£238.50 - £361.50

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Anti-EGFR antibody (424-605aa) [AT6E3] (STJA0041600)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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Anti-EGFR antibody (424-605aa) [AT6E3] (STJA0041921)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£238.50 - £361.50

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Western blot analysis of various cells using EGFR Polyclonal Antibody diluted at 1：1000

Anti-EGFR antibody (661-710 aa) (STJ92850)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-EGFR antibody (800-900) [S9MR] (STJ11102229)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded Carcinoma of Human lung tissue using EGFR antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-EGFR antibody (900-1210 aa) [1B10] (STJA0013541)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£224.50 - £308.50

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Immunofluorescence analysis of human-liver tissue. 1, EGFR Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-EGFR antibody (986-1035 aa) (STJ92862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-EGFR antibody (N-Term) (STJA0005395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£319.50

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Anti-EGFR antibody (N-Term) (STJA0041200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£474.50

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Anti-EGFR antibody [1A11-G9-1E9] (STJA0033223)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-EGFR antibody [2+00E+09] (STJ16100028)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IP/EM

Reactivity :

Human

£260.50

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Anti-EGFR antibody [3A9-9D2-9F7] (STJA0032850)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Western blot detection of EGFR in A549, MDA-MB-468 and COS7 cell lysates using EGFR mouse mAb (dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.

Anti-EGFR antibody [5E10-G10-E11] (STJ99229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human/Monkey

£149.00 - £360.00

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Anti-EGFR antibody [5E10-G10-E11] (STJA0033224)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Monkey

£133.50 - £281.50

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Anti-EGFR antibody [5E9-10B8-8B9] (STJA0032805)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-EGFR antibody [6H11-2D11-G3] (STJA0033225)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Monkey

£133.50 - £281.50

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Anti-EGFR antibody [BP6097] (STJA0037647)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£244.50 - £335.50

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Anti-EGFR antibody [BP6098] (STJA0037648)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£337.50

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Anti-EGFR antibody [DM128] (STJA0038162)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/ELISA

Reactivity :

Human

£432.50

Choose Options

Anti-EGFR antibody [EGFR1] (STJ16103137)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/IHC-F/ICC

Reactivity :

Horse/Human

£532.50

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Anti-EGFR antibody [IHC565] (STJA0042040)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-p

Reactivity :

Human

£152.50 - £467.50

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Anti-EGFR antibody [PT0520R] (STJA0039453)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-EGFR antibody [R03-7I3] (STJA0036681)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

Choose Options