Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of various lysates using PSMD14 Rabbit mAb (STJ11102503) at 1:1000 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-PSMD14 antibody (112-210) [S3MR] (STJ11102503)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PSMD14 antibody (152-202 aa) (STJ195728)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunofluorescence analysis of NIH-3T3 cells using PSMD14 Rabbit polyclonal antibody (STJ112678) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PSMD14 antibody (160-300) (STJ112678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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PSMD14 antibody western blot HeLa lysates STJA0037073

Anti-PSMD14 antibody [R08-3T6] (STJA0037073)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Immunofluorescence analysis of HeLa cells using PSMD2 antibody (STJ25186). Blue: DAPI for nuclear staining.

Anti-PSMD2 antibody (1-260) (STJ25186)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of various cells using PSMD2 Polyclonal Antibody

Anti-PSMD2 antibody (101-150 aa) (STJ95253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-PSMD3 antibody (351-400 aa) (STJ95255)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using PSMD3 antibody (STJ25187) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PSMD3 antibody (700-800) (STJ25187)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PSMD6 antibody (201-250 aa) (STJ94882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from Rat brain, using PSMD6 Rabbit polyclonal antibody (STJ11100219) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PSMD6 antibody (23-307) (STJ11100219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of MCF-7 cells using PSMD7 antibody (STJ27309). Blue: DAPI for nuclear staining.

Anti-PSMD7 antibody (1-324) (STJ27309)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Anti-PSMD7 antibody (STJA0008403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PSMD8 antibody (1-51 aa) (STJ195541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunofluorescence analysis of C6 cells using PSMD8 Rabbit polyclonal antibody (STJ29035) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PSMD8 antibody (101-350) (STJ29035)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using PTEN antibody (STJ113730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PTEN antibody (1-200) (STJ113730)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of NIH/3T3 cells using PTEN antibody (STJ29874) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PTEN antibody (190-304) (STJ29874)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates using PTEN Mouse monoclonal antibody (STJ112974) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PTEN antibody (204-403) [S4MM] (STJ112974)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunofluorescence analysis of human-heart tissue. 1, PTEN Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-PTEN antibody (251-300 aa) (STJ98583)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-PTEN (PT2124) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: HEK293 Lane 3: MCF7Predicted band size: 47kDaObserved band size: 47kDa

Anti-PTEN antibody (300-403) [PT2124] (STJA0006236)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using PTEN Rabbit monoclonal antibody antibody (STJ11104594) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PTEN antibody (304-403) [S4594RM] (STJ11104594)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Anti-PTEN antibody IHC human endometrioid adenocarcinoma STJ180622

Anti-PTEN antibody (Full Length) [ZR235] (STJ180622)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$217.35 - $871.47

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Anti-PTEN antibody (STJA0038042)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$258.75 - $388.47

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PTEN antibody immunohistochemistry human colon carcinoma STJA0032918

Anti-PTEN antibody [7H2-6G9-6H5] (STJA0032918)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$184.23 - $388.47

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Anti-PTEN antibody [IHC110] (STJA0042253)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-p

Reactivity :

Human

$315.33 - $1,176.45

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Anti-PTEN antibody [PT0226R] (STJA0039660)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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PTEN antibody western blot K562 C6 3T3 HeLa STJA0034771

Anti-PTEN antibody [R02-9D3] (STJA0034771)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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PTEN antibody western blot HeLa NIH/3T3 C6 STJA0036141

Anti-PTEN antibody [R08-4A4] (STJA0036141)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-PTEN antibody [SQab30363] (STJ16103072)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$977.73

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Anti-PTEN antibody [SRM13058] (STJ11107788)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot human Jurkat cells PTP1B detection with and without calyculin A treatment STJA0041316

Anti-PTP1B antibody (C-Term) [M234] (STJA0041316)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC

Reactivity :

Human

$799.71

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Western blot PTP1B phospho-Tyr152 detection in treated and untreated mouse SYF cells STJA0041342

Anti-PTP1B antibody (N-Term) (STJA0041342)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-PTP1B antibody [1D10] (STJA0032701)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Monkey/Rat

$309.81 - $425.73

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PTP1B antibody western blot HeLa cells STJA0036245

Anti-PTP1B antibody [R02-2E3] (STJA0036245)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human

$184.23 - $388.47

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PTP1B antibody western blot 293T HCT116 STJA0034773

Anti-PTP1B antibody [R05-3B9] (STJA0034773)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

$184.23 - $388.47

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Anti-PTP1B antibody [SRM14584] (STJ11109314)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Western blot analysis of various cells using PTP1B Polyclonal Antibody diluted at 1：500

Anti-PTPN1 antibody (16-65 aa) (STJ95265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Western blot analysis of lysates from wild type (WT) and PTPN1 knockout (KO) HeLa (KO) cells, using [KO Validated] PTPN1 Rabbit polyclonal antibody (STJ11104770) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PTPN1 antibody (281-435) (STJ11104770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$174.57 - $853.53

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Immunofluorescence analysis of U2OS cells using PTPN1 Rabbit polyclonal antibody (STJ28044) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PTPN1 antibody (281-435) (STJ28044)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-PTPN1 antibody (C-Term) [M234] (STJA0005551)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

$544.41

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Anti-PTPN1 antibody (N-Term) (STJA0005552)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$507.15

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Confocal imaging of NIH/3T3 cells using SHP2 Rabbit monoclonal antibody (STJ11101649, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-PTPN11 antibody (1-100) [S9MR] (STJ11101649)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunoprecipitation analysis of 300 Mu g extracts of MCF7 cells using 3 Mu g SHP2 antibody (STJ114360). Western blot was performed from the immunoprecipitate using SHP2 antibody (STJ114360) at a dilution of 1:2000.

Anti-PTPN11 antibody (1-460) (STJ114360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of various cells using SH-PTP2 Polyclonal Antibody diluted at 1：2000

Anti-PTPN11 antibody (321-370 aa) (STJ95660)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Western blot analysis of various cells using SH-PTP2 Polyclonal Antibody diluted at 1：1000

Anti-PTPN11 antibody (508-557 aa) (STJ95659)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of PC-12 cells using SHP2 Rabbit polyclonal antibody (STJ25224) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-PTPN11 antibody (514-593) (STJ25224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-PTPN11 antibody (546-595 aa) (STJ95658)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-PTPN11 antibody (N-Term) [M163] (STJA0005583)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

$440.91

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Western blot analysis of extracts of various cell lines, using PTPN4 antibody (STJ26538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-PTPN4 antibody (1-240) (STJ26538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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