Disease | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using CDC42 antibody (STJ11101566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDC42 antibody (60-191) [S6MR] (STJ11101566)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CDC42 antibody (80-160 aa) (STJ191678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-CDC42 antibody [PT1501R] (STJA0040263)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of extracts of various cell lines, using CDC5L antibody (STJ27506) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CDC5L antibody (563-802) (STJ27506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of CDC5L Antibody. The lane on the right is blocked with the CDC5L peptide.

Anti-CDC5L antibody (721-770 aa) (STJ92180)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-CDC5L antibody [SRM13670] (STJ11108400)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£0.00

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Western blot analysis of various lysates, using HRPT2/ CDC73 Rabbit monoclonal antibody (STJ11105154) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CDC73 antibody (300-400) [S5154RM] (STJ11105154)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from mouse testis, using HRPT2/ CDC73 Rabbit polyclonal antibody (STJ11103808). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CDC73 antibody (435-505) (STJ11103808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of 1, mouse-liver 2, mouse-brain cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-CDC73 antibody (51-100 aa) (STJ98854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of E-Cadherin in paraffin-embedded mouse liver using E-Cadherin Rabbit polyclonal antibody (STJ11105677) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CDH1 antibody (373-622) (STJ11105677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of HCT116, using E-Cadherin antibody (STJ113165) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CDH1 antibody (373-622) (STJ113165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of paraffin-embedded mouse kidney using E-Cadherin Mouse monoclonal antibody (STJ11100105) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-CDH1 antibody (373-622) [S5MM] (STJ11100105)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£126.50 - £554.50

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Human Breast carcinoma tissue was stained with E-cadherin (ABT181) Antibody

Anti-CDH1 antibody (700-800) [ABT181] (STJA0006294)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/ELISA

Reactivity :

Human/Mouse/Rat

£149.00 - £242.00

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Human Breast tissue was stained with E-cadherin (ABT270) Antibody

Anti-CDH1 antibody (700-800) [ABT270] (STJA0006293)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/ELISA

Reactivity :

Human

£149.00 - £242.00

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Anti-CDH1 antibody (C-Term) (STJA0005284)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot detection of E-Cadherin in T47D, HCT116, MDA-MB-468 and Caco-2 cell lysates using E-Cadherin mouse mAb (dilution 1:2000).Predicted band size:135kDa.Observed band size:135kDa.

Anti-CDH1 antibody [6B10-F4-G10] (STJ99316)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis using E-cadherin monoclonal antibody against LNCAP (1) , A431 (2) , DU145 (3) , PC-3 (4) , MCF-7 (5) , PC-12 (6) , NIH/3T3 (7) , C6 (8) and COS7 (9) cell lysate.

Anti-CDH1 antibody [7H12] (STJ98009)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Monkey

£149.00 - £360.00

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Anti-CDH1 antibody [IHC564] (STJA0041997)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-p

Reactivity :

Human

£135.50 - £374.50

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Immunohistochemistry analysis of CDK19 in paraffin-embedded rat testis using CDK19 Rabbit polyclonal antibody (STJ118549) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDK19 antibody (250-350) (STJ118549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-CDK19 antibody (30-110 aa) (STJ191675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using CDK2 rabbit monoclonal antibody (STJ11101225) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDK2 antibody (199-298) [S5MR] (STJ11101225)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of CDK2 in paraffin-embedded human esophageal cancer using [KO Validated] CDK2 Rabbit polyclonal antibody (STJ113540) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDK2 antibody (200-298) (STJ113540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from wild type (WT) and CDK2 knockout (KO) 293T cells, using [KO Validated] CDK2 Rabbit pAb (STJ119973) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-CDK2 antibody (200-298) (STJ119973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, Cdk2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). NSE monoclonal antibody (13E2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CDK2 antibody (231-280 aa) (STJ92197)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Cdk2 antibody [5B12D1] (STJ16103574)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IHC/WB

Reactivity :

Human/Mouse/Rat

£746.50

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Anti-Cdk2 antibody [6D5B5] (STJ16103575)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£746.50

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Anti-CDK2 antibody [PT0233R] (STJA0039631)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-CDK4 antibody (1-303aa) [AT6D10] (STJA0041666)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human

£219.50 - £332.50

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Immunofluorescence analysis of Hela cell. 1, Cdk4 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-CDK4 antibody (1-50 aa) (STJ96563)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using [KO Validated] CDK4 Rabbit pAb (STJ23055) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-CDK4 antibody (200-303) (STJ23055)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-CDK4 antibody (204-303 aa) [SRM5126] (STJ11106945)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Immunoprecipitation analysis of 300 Mu g extracts of Hela cells using 3 Mu g [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11101180). Western blot was performed from the immunoprecipitate using [KO Validated] CDK4 Rabbit polyclonal antibody (STJ11101180) at a dilition of 1:1000.

Anti-CDK4 antibody (204-303) (STJ11101180)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates, using CDK4 rabbit monoclonal antibody (STJ11101279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDK4 antibody (204-303) [S9MR] (STJ11101279)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£143.50 - £618.50

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Western blot detection of CDK4 in Hela, Lncap and F9 cell lysates using CDK4 mouse mAb (1:1000 diluted).Predicted band size:34KDa.Observed band size:33KDa.

Anti-CDK4 antibody [3F9-B12-C8] (STJ99110)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£149.00 - £360.00

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Anti-Cdk4 antibody [DCS-31] (STJ16100131)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICC/IF/WB/FC/IP

Reactivity :

Human/Mouse/Rat

£1,025.50

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Anti-CDK4 antibody [EP180] (STJ16101441)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£1,486.50

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Anti-CDK4 antibody [IHC077] (STJA0042132)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-p

Reactivity :

Human

£188.50 - £580.50

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Anti-CDK4 antibody [PT0399R] (STJA0039565)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-CDK4 antibody [SQab1864] (STJ16102878)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/ICC/IF/IHC-P/IP/WB

Reactivity :

Human

£708.50

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Anti-CDK4 antibody [SRM13318] (STJ11108048)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunoprecipitation analysis of 900 Mu g extracts of A-549 cells using 3 Mu g CDK5 antibody (STJ28297). Western blot was performed from the immunoprecipitate using CDK5 antibody (STJ28297) at a dilution of 1:1000.

Anti-CDK5 antibody (1-292) (STJ28297)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of HepG2 cells using Cdk5 Polyclonal Antibody diluted at 1：500

Anti-CDK5 antibody (1-50 aa) (STJ92200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from wild type (WT) and CDK5 knockout (KO) 293T cells, using [KO Validated] CDK5 Rabbit polyclonal antibody (STJ11100053) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDK5 antibody (170-289) (STJ11100053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of Hela, using CDK5 rabbit monoclonal antibody (STJ11101567) at 1:1000 dilutionSecondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CDK5 antibody (193-292) [S7MR] (STJ11101567)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of lysates from wild type (WT) and CDK5 knockout (KO) 293T cells, using [KO Validated] CDK5 Rabbit monoclonal antibody (STJ11105132) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDK5 antibody (92-289 aa) [S5132RM] (STJ11105132)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot detection of CDK5 (N-terminus) in 3T3, Hela, PC-12, COS7, Jurkat and K562 lysates using Cdk5 mouse mAb (1:500 diluted).Predicted band size: 36KDa.Observed band size: 36KDa.Exposure time:5min.

Anti-CDK5 antibody (N-Term) [2E8-F9-B7-C11] (STJ99113)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat/Monkey

£149.00 - £360.00

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Western blot analysis of1) Hela Cell, 2) Jurkat Cell, 3) Mouse Brain Tissue, 4) Rat Brain Tissue lysate using mouse monoclonal antibody diluted at 1:2,000.

Anti-CDK5 antibody [3D1] (STJ196638)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Anti-CDK5 antibody [DC17+DC34] (STJA0005342)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human/Mouse/Rat

£319.50

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