Developmental Biology | Research Area | St John's Laboratory

Immunofluorescence analysis of rat-lung tissue. 1, p53 (phospho Ser15) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-TP53-Ser15 antibody (1-50 aa) (STJ90365)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP/IF/ELISA

Reactivity :

Human/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-p53 (S20) Polyclonal Antibody

Anti-Phospho-TP53-Ser20 antibody (10-59 aa) (STJ90366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-p53 (S315) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-TP53-Ser315 antibody (281-330 aa) (STJ90367)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-p53 (S33) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-TP53-Ser33 antibody (1-50 aa) (STJ90368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of HELA cells using Phospho-p53 (S366) Polyclonal Antibody

Anti-Phospho-TP53-Ser366 antibody (331-380 aa) (STJ90933)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of HELA Cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-TP53-Ser366 antibody (STJ98965)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-p53 (S37) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-TP53-Ser37 antibody (11-60 aa) (STJ90441)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using p53 (Phospho-Ser376) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-TP53-Ser376 antibody (344-393 aa) (STJ91191)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from Jurkat cells treated with UV 15', using p53 (Phospho-Ser378) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-TP53-Ser378 antibody (344-393 aa) (STJ90574)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p53 (Phospho-Ser392) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-TP53-Ser392 antibody (344-393 aa) (STJ91044)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£49.00 - £261.00

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Western blot of rat brain nuclear fraction lysate showing specific immunolabeling of the ~53 kDa p53 phosphorylated at Ser392 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-TP53-Ser392 antibody (STJA0003735)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£488.50

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Western blot analysis of various cells using Phospho-p53 (S46) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-TP53-Ser46 antibody (11-60 aa) (STJ90369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Monkey

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-p53 (S6) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-TP53-Ser6 antibody (1-50 aa) (STJ90370)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Monkey

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-p53 (S9) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-TP53-Ser9 antibody (1-50 aa) (STJ90371)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£49.00 - £261.00

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Western blot analysis of lysates from MDA cells, using Phospho-p53-T18 Rabbit pAb (STJ22441). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-TP53-T18 antibody (STJ22441)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from 293T cells, using Phospho-p53-T55 Rabbit mAb (STJ11102577) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-TP53-T55 antibody [S7MR] (STJ11102577)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunofluorescence analysis of rat-lung tissue. 1, p53 (phospho Thr18) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-TP53-Thr18 antibody (1-50 aa) (STJ90372)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p53 (Phospho-Thr387) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-TP53-Thr387 antibody (344-393 aa) (STJ91190)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using p53 (Phospho-Thr81) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-TP53-Thr81 antibody (51-100 aa) (STJ91132)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-TP63-Ser160/162 antibody (STJ196371)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from LOVO cells treated with nocodazole 1ug/ml 18h, A549 cells treated with nocodazole 1ug/ml 18h and HepG2 cells treated with nocodazole 1ug/ml 18h, using p63 (Phospho-Ser395) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-TP63-Ser395 antibody (361-410 aa) (STJ90646)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-p63 (S455) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-TP63-Ser455 antibody (421-470 aa) (STJ90859)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-TUBA1C-Ser-165 antibody (STJA0005627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£367.50

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Anti-Phospho-TUBB3-Ser-172 antibody (STJA0005631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£367.50

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Western Blot analysis of 1 HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-USF1-Thr153 antibody (STJA0009960)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HEL cells using Phospho-VASP-S157 Rabbit pAb (STJ116383) at 1:1000 dilution. HEL cells were treated by Forskolin (20 uM) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 0. 8s.

Anti-Phospho-VASP-S157 antibody (STJ116383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-VASP-S157 Rabbit monoclonal antibody (STJ11105906) at 1:4000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-VASP-S157 antibody [S5906RM] (STJ11105906)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from HEL cells, using Phospho-VASP-S157 Rabbit monoclonal antibody (STJ11105907) at 1:3000 dilution. HEL cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-VASP-S157 antibody [S5907RM] (STJ11105907)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysates from C6 cells, using Phospho-VASP-S239 Rabbit pAb (STJ22444) at 1:1000 dilution. C6 cells were treated by Forskolin (10 uM) at 37 °C for 1 hour after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-VASP-S239 antibody (STJ22444)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-VASP-S239 antibody [SRM13699] (STJ11108429)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£0.00

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Western blot analysis of various cells using Phospho-VASP (S157) Polyclonal Antibody

Anti-Phospho-VASP-Ser157 antibody (124-173 aa) (STJ90431)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human tonsil. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-VASP-Ser157 antibody (STJ90777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from RAW264.7 cells, using VASP (Phospho-Ser238) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-VASP-Ser238 antibody (206-255 aa) (STJ90432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-VASP-Ser239 antibody (STJ196466)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-VASP-Ser239 antibody [PT1281R] (STJA0040080)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-VASP-Thr-278 antibody (STJA0005634)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-Phospho-VASP-Thr-278 antibody (STJA0041443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-VASP-Thr278 antibody (STJ91280)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of NIH/3T3 cells, using VAV2 (Phospho-Tyr142) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-VAV2-Tyr142 antibody (108-157 aa) (STJ91109)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of JK cells using Phospho-Vav3 (Y173) Polyclonal Antibody

Anti-Phospho-VAV3-Tyr173 antibody (STJ90999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Vimentin-S39 Rabbit pAb (STJ117904) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37 °C for 20 hours. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 1s.

Anti-Phospho-VIM-S39 antibody (STJ117904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S56 pAb (STJ117902) at 1:3000 dilution or Vimentin antibody (STJ249342). HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. HeLa cells were treated by Paclitaxel (100 nM/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-VIM-S56 antibody (STJ117902)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Vimentin-S83 Rabbit pAb (STJ11101153) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37 °C for 20 hours. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-VIM-S83 antibody (STJ11101153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S83 pAb (STJ117897) at 1:2000 dilution or Vimentin antibody (STJ26092). HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours or treated by Paclitaxel (100 nM/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-VIM-S83 antibody (STJ117897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

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Anti-Phospho-VIM-Ser39 antibody (STJ196472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of A549 cells using Phospho-Vimentin (S56) Polyclonal Antibody

Anti-Phospho-VIM-Ser56 antibody (31-80 aa) (STJ90927)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of 293 cells using Phospho-Vimentin (S83) Polyclonal Antibody

Anti-Phospho-VIM-Ser83 antibody (56-105 aa) (STJ90926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of HELA cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000

Anti-Phospho-VIM-Tyr38 antibody (STJ98572)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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