Recombinant antibodies

Recombinant research antibodies provide consistent, animal-free, and highly specific binding reagents for a wide range of applications. Using advanced recombinant antibody discovery platforms, such as phage display and single B-cell antibody technology, our high-quality recombinant monoclonal antibodies are tailored for research use. In phage display, antibody fragments like single-chain variable fragment (scFv) are displayed on bacteriophages, allowing rapid selection from vast immune or naïve libraries, without the variability of animal immunisation. Single B-cell technology isolates and sequences antibody genes from individual B cells, preserving native heavy and light chain pairing for optimal specificity.

Our recombinant research antibodies are produced using optimised expression systems, including mammalian cells like CHO and HEK for full-length antibodies with correct folding and post-translational modifications. Following production, each batch undergoes stringent purification and characterisation to ensure reproducible performance in techniques, including western blot, immunofluorescence, flow cytometry and ELISA.

Featured recombinant antibodies


Recombinant Antibodies for Cell Biology

Recombinant antibodies are essential tools in cell biology, particularly for observing and quantifying dynamic cellular processes in real time. The stability, specificity and consistency across different batches make recombinant antibodies ideal for live-cell imaging and tracking to study activities like protein trafficking, cytoskeletal rearrangements, cell division and receptor-ligand interactions. Cytoskeleton, organelles, and stress pathways for imaging and WB.

TargetApplicationNotesBrowse
β-Actin (ACTB)WB loading control~42 kDa cytoskeletal markerACTB recombinant antibodies
α/β-TubulinWB/IF cytoskeletonMicrotubule networkTubulin recombinant antibodies
Lamin A/C (LMNA/C)Nuclear envelopeOrganelle identity markerLamin recombinant antibodies
HSP70 (HSPA1A)Stress responseChaperone; heat shock markerHSP70 recombinant antibodies

Recombinant Antibodies for Immunology

Uncover the dynamic dance between immune cells by visualising crucial interactions like the formation of the immunological synapse between T-cells and B-cells, providing clarity and precision at the cellular level. Additionally, these antibodies enable the accurate tracking of immune cell migration towards sites of inflammation, offering vital insights into how the immune system responds to infection and disease.

Achieve reliable and reproducible results every time, thanks to their superior specificity, high sensitivity, and batch-to-batch consistency, making them perfect for a wide range of applications from flow cytometry to immunofluorescence.

TargetUseNotesBrowse
CD3 / CD4 / CD8T-cell phenotyping (FC/IF)Pan-T and subset markersT-cell marker recombinant antibodies
CCL3 (MIP-1α)Chemokine profilingInflammation & migrationCCL3 recombinant antibodies
PD-1 / PD-L1Checkpoint studiesExhaustion & immunotherapyPD-1/PD-L1 recombinant antibodies

Recombinant Antibodies for Neuroscience

Engineered to be the ideal tool for visualising key events in immunology, offering exceptional specificity and sensitivity. Recombinant antibodies allow researchers to uncover the dynamic dance between immune cells by visualising crucial interactions like the formation of the immunological synapse between T-cells and B-cells, providing clarity and precision at the cellular level. Additionally, these antibodies enable the accurate tracking of immune cell migration towards sites of inflammation, offering vital insights into how the immune system responds to infection and disease.

With our recombinant antibodies, you can achieve reliable and reproducible results every time, thanks to their superior specificity, high sensitivity, and batch-to-batch consistency, making them perfect for a wide range of applications from flow cytometry to immunofluorescence.

TargetUseNotesBrowse
NeuN (RBFOX3)Neuron ID (IHC/IF)Loss indicates neuronal damageNeuN recombinant antibodies
GFAPAstrocyte markerGliosis & neuroinflammationGFAP recombinant antibodies
Iba1 (AIF1)Microglia activationImmune response in CNSIba1 recombinant antibodies
SynaptophysinSynapse densityPresynaptic vesiclesSYP recombinant antibodies
Amyloid-β (Aβ40)AD modelsPlaques/aggregatesAβ40 recombinant antibodies

Recombinant Antibodies for Oncology

Understanding the mechanisms that drive cancer progression is essential for developing effective therapies. Our recombinant research antibodies provide reliable and specific tools for studying key processes in oncology, including the invasion and metastasis of cancer cells, the effects of therapeutic antibodies on tumour growth and signaling pathways, and the molecular mechanisms of cell death such as apoptosis and autophagy. Designed for consistency and reproducibility, recombinant antibodies enable tracking of dynamic changes in tumour biology, investigate drug responses, and uncover new therapeutic targets.

TargetAxis/UseNotesBrowse
BCL-2ApoptosisPro-/anti-apoptotic balanceBCL-2 recombinant antibodies
BAXApoptosisPro-/anti-apoptotic balanceBAX recombinant antibodies
Caspase-1/7ApoptosisPro-/anti-apoptotic balanceCASP1/7 recombinant antibodies
AKTAKTTotal + phospho readoutsAKT recombinant antibodies
p-AKT (S473)PI3KTotal + phospho readoutspAKT recombinant antibodies
MEK1MAPKStress & growth signalsMAPK recombinant antibodies
p38 (p-T180/Y182)MAPKStress & growth signalsMAPK recombinant antibodies
EGFRBiomarkersSurface & secreted markersEGFR recombinant antibodies
EpCAMBiomarkersSurface & secreted markersEpCAM recombinant antibodies
MUC1BiomarkersSurface & secreted markersMUC1 recombinant antibodies
CA9BiomarkersSurface & secreted markersCA9 recombinant antibodies
p53 (TP53)Tumour suppressorDNA damage responsep53 recombinant antibodies

Recombinant Antibodies for DNA Repair & Genomic Stability

Defects in DNA repair pathways are a significant driver of genomic instability and cancer. Recombinant antibodies against key mismatch repair proteins provide reliable tools for research. These antibodies enable researchers to accurately assess the status of these crucial DNA repair proteins, providing critical insights for advancing research.

TargetPathwayNotesBrowse
MLH1MMRMarker for MSI testing workflowsMLH1 recombinant antibodies
PMS2MMRPairs with MLH1 for panelsPMS2 recombinant antibodies

Recombinant Antibody Formats

Recombinant antibodies are engineered proteins produced from defined DNA sequences, offering consistent quality, high specificity, and reproducibility for research applications. Unlike traditional hybridoma-derived antibodies, recombinant antibodies are fully characterized and free from lot-to-lot variability, making them ideal for sensitive assays and advanced imaging studies. Pick the format that matches your assay and detection system.

FormatBest forNotesBrowse
Full-length IgGWB, IF/IHC, FC, ELISA, IPNative effector & bivalencyRecombinant IgG
scFvImaging, capture, HCISmall size; no Fc interactionsscFv recombinant antibodies
Fab / fragmentsReduced steric hindranceImproved tissue penetrationFab fragments
Tagged/ConjugatedDirect IF/FC/WBAF dyes, PE/APC, HRP/APConjugated

Advantages

Recombinant antibodies have improved yield, quality and homogeneity by eliminating process variables such as hybridoma instability and supply inconsistencies. Our recombinant antibodies offer researchers a dependable solution for accurate, reproducible results in discovery and validation workflows. The benefits of recombinant antibodies are clear in their consistent performance and animal-free production. We use advanced recombinant antibody technology to create high-quality products.

AdvantageDetailsNotes
ReproducibilityFixed sequence; controlled expressionEliminates hybridoma drift
SpecificityLibrary selection or native B-cell pairingReduced off-target binding
EngineeringEasy Fc, scFv, Fab, tag/dye fusionsCustom formats for assays
Ethics & supplyAnimal-free; scalable manufacturingConsistent availability

High-Content Imaging (HCI)

Large datasets demand sequence-defined probes for reproducibility, multiplexing, and high specificity, which are key reasons HCI labs favour recombinant antibodies. This technique is widely used in various research fields, particularly in drug discovery and basic research that require a quantitative and high-throughput approach.

HCI utilizes automated microscopes and image analysis software to simultaneously measure multiple parameters within individual cells, such as protein localization, cell shape, nuclear size, and cytoskeletal integrity. This is done across thousands of cells in a single experiment.

NeedWhy recombinant helpsNotes
ReproducibilityFixed sequence; identical lotsReduces assay-to-assay noise
MultiplexingCustom dye/tagged variantsPanel building without cross-reactivity
SpecificityRigorous selection/validationCleaner signals for quantitation

Validation & QC

Each recombinant lot ships with defined specs and is amenable to stringent functional validation. Review affinity, specificity, epitope details, conjugation/format, and storage guidance on the COA/datasheet.

AttributeTypical infoWhy it matters
Affinity (KD)Low nM–pM (target-dependent)Sensitivity & retention
SpecificityCompetition & cross-reactivity panelsMinimises off-target signal
EpitopeLinear vs conformationalGuides fixation/denaturation
Conjugate/formatAF/PE/APC/HRP/APOne-step detection vs secondary
StorageLyophilised or −20 to −80 °CPreserves activity

Recombinant vs Hybridoma (Quick Compare)

FeatureRecombinantHybridoma-derived
Lot consistencyHigh (fixed sequence)Variable (clone drift)
EngineeringFlexible (Fc/scFv/Fab/tags)Limited
Animal useAnimal-free productionAnimal immunisation
Scale & supplyScalable expressionHybridoma stability dependent

FAQs

QuestionAnswer
How are recombinant antibodies different from hybridoma products?They are produced from a defined sequence in an expression system, giving consistent lots and enabling custom formats (Fc, scFv, tags) without hybridoma drift.
Do recombinant antibodies work in fixed tissues?Yes they can. We recommend checking the epitope and recommended fixation. Conformational epitopes may require mild retrieval or optimized permeabilisation.
Can I get the same clone in multiple conjugates?We can provide a range of conjugation options for recombinant antibodies bought in bulk >1mL. Please enquire for further details.

Need help choosing a recombinant antibody?

Tell us your target, application (WB/IF/FC/ELISA/IP), species, and any conjugation needs. We’ll recommend clones and share lot-specific QC.

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